Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4026445..4027064 Replicon chromosome
Accession NZ_CP067255
Organism Klebsiella pneumoniae strain ESBL_DR09

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag JJB15_RS19530 Protein ID WP_002892050.1
Coordinates 4026846..4027064 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag JJB15_RS19525 Protein ID WP_002892066.1
Coordinates 4026445..4026819 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB15_RS19515 (4021597) 4021597..4022790 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JJB15_RS19520 (4022813) 4022813..4025959 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
JJB15_RS19525 (4026445) 4026445..4026819 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
JJB15_RS19530 (4026846) 4026846..4027064 + 219 WP_002892050.1 HHA domain-containing protein Toxin
JJB15_RS19535 (4027223) 4027223..4027789 + 567 WP_002892030.1 maltose O-acetyltransferase -
JJB15_RS19540 (4027761) 4027761..4027901 - 141 WP_004147370.1 hypothetical protein -
JJB15_RS19545 (4027922) 4027922..4028392 + 471 WP_002892026.1 YlaC family protein -
JJB15_RS19550 (4028367) 4028367..4029818 - 1452 WP_032435563.1 PLP-dependent aminotransferase family protein -
JJB15_RS19555 (4029919) 4029919..4030617 + 699 WP_032435564.1 GNAT family protein -
JJB15_RS19560 (4030614) 4030614..4030754 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
JJB15_RS19565 (4030754) 4030754..4031017 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T185948 WP_002892050.1 NZ_CP067255:4026846-4027064 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T185948 NZ_CP089068:c476810-476532 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT185948 WP_002892066.1 NZ_CP067255:4026445-4026819 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT185948 NZ_CP089068:c476520-476215 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAATGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCAGGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References