Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 109858..110383 Replicon plasmid pESBL_DR28a
Accession NZ_CP067251
Organism Escherichia coli strain ESBL_DR28

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag JJB16_RS23555 Protein ID WP_001159871.1
Coordinates 109858..110163 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag JJB16_RS23560 Protein ID WP_000813630.1
Coordinates 110165..110383 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB16_RS23540 (105820) 105820..106986 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
JJB16_RS23545 (107574) 107574..108329 - 756 WP_000852146.1 replication initiation protein RepE -
JJB16_RS23550 (109051) 109051..109857 - 807 WP_000016970.1 site-specific integrase -
JJB16_RS23555 (109858) 109858..110163 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
JJB16_RS23560 (110165) 110165..110383 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
JJB16_RS23565 (110943) 110943..111173 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
JJB16_RS23570 (111170) 111170..111586 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
JJB16_RS23575 (111661) 111661..113226 + 1566 WP_001128474.1 AAA family ATPase -
JJB16_RS23580 (113211) 113211..114233 + 1023 WP_000361402.1 helicase UvrD -
JJB16_RS23585 (114487) 114487..115173 - 687 Protein_148 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul1 / qacE / aadA5 / mph(A) / erm(B) / aac(3)-IId / fosA3 / blaCTX-M-65 / sitABCD iutA / iucD / iucC / iucB / iucA 1..133432 133432
- flank IS/Tn - - 114487..114834 347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T185931 WP_001159871.1 NZ_CP067251:c110163-109858 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T185931 NZ_CP089065:1754415-1754693 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT185931 WP_000813630.1 NZ_CP067251:c110383-110165 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT185931 NZ_CP089065:1754705-1755010 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References