Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3758836..3759490 Replicon chromosome
Accession NZ_CP067250
Organism Escherichia coli strain ESBL_DR28

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag JJB16_RS17810 Protein ID WP_000244781.1
Coordinates 3758836..3759243 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JJB16_RS17815 Protein ID WP_000354046.1
Coordinates 3759224..3759490 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB16_RS17790 (3754793) 3754793..3756526 - 1734 WP_021523238.1 single-stranded-DNA-specific exonuclease RecJ -
JJB16_RS17795 (3756532) 3756532..3757242 - 711 WP_001521231.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JJB16_RS17800 (3757267) 3757267..3758163 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
JJB16_RS17805 (3758275) 3758275..3758796 + 522 WP_001521233.1 flavodoxin FldB -
JJB16_RS17810 (3758836) 3758836..3759243 - 408 WP_000244781.1 protein YgfX Toxin
JJB16_RS17815 (3759224) 3759224..3759490 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JJB16_RS17820 (3759733) 3759733..3760713 + 981 WP_000886050.1 tRNA-modifying protein YgfZ -
JJB16_RS17825 (3760790) 3760790..3761449 - 660 WP_000250269.1 hemolysin III family protein -
JJB16_RS17830 (3761613) 3761613..3761924 - 312 WP_001182949.1 N(4)-acetylcytidine aminohydrolase -
JJB16_RS17835 (3761969) 3761969..3763402 + 1434 WP_001521235.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T185921 WP_000244781.1 NZ_CP067250:c3759243-3758836 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T185921 NZ_CP089063:c4287480-4287181 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT185921 WP_000354046.1 NZ_CP067250:c3759490-3759224 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT185921 NZ_CP089063:c4287184-4286894 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References