Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 289188..289685 Replicon chromosome
Accession NZ_CP067250
Organism Escherichia coli strain ESBL_DR28

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JJB16_RS01310 Protein ID WP_001173343.1
Coordinates 289512..289685 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag JJB16_RS01305 Protein ID WP_000398619.1
Coordinates 289188..289484 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB16_RS01285 (285919) 285919..286638 - 720 WP_000611288.1 two-component system response regulator DcuR -
JJB16_RS01290 (286635) 286635..288266 - 1632 WP_001216477.1 sensor histidine kinase -
JJB16_RS01295 (288447) 288447..288677 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
JJB16_RS01300 (288689) 288689..288961 + 273 WP_000405651.1 GNAT family N-acetyltransferase -
JJB16_RS01305 (289188) 289188..289484 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JJB16_RS01310 (289512) 289512..289685 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JJB16_RS01315 (289804) 289804..291321 - 1518 WP_001295074.1 lysine--tRNA ligase -
JJB16_RS01320 (291558) 291558..293015 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T185902 WP_001173343.1 NZ_CP067250:289512-289685 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T185902 NZ_CP089055:c6708-6313 [Acinetobacter ursingii]
ATGATTTACCTCTTAGACACAAATATTTGTATCTATGTGATCAATCATAAGCCAGAGCATGTTTTTGAACGCTTTAAACA
ATACCAGCTTGGTCAGCTTGCGATTTCAAGCATAACAGCATCCGAATTAGCTTTTGGCGTTGAAAAATCTGGCTCTGAGC
GTAACAAACAGGCATTGAACAAGTTTTTATCACCCTTAGATATTTTACCTTACGATGAGAAAGCAATCTGGCATTACGCA
AAACTACGTCAAGATCTACAATCTACAGGTAAAATAATTGGTAGTTTGGATATGCTAATTGCAGCGCATGCCCTAGCTTT
AGATGTTGTATTAGTCACAAATAACATCAAGGAATTTGAGCGTATAGATGGATTAAAGTTAGAAAACTGGGTTTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT185902 WP_000398619.1 NZ_CP067250:289188-289484 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT185902 NZ_CP089055:c6935-6705 [Acinetobacter ursingii]
ATGGAAGTTGCTAAAGTCTTTCAAACAGGTCGAAGTCAAGCCGTTAGGTTACCTAAAGCATTTCGTTTTAATGGTACTGA
AGTAGCGATTAAAAGTTTTGGACGTGGTGTGTTATTGATGCCTATCGACAATCCTTGGGATGTAATGCTAGAAGCTCTAA
ACGAGTTTGAAGTTGGATTCAAGTTAGAACGGGCAGATCAGGGCGAACAGGTACGTGAGGATTTCAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References