Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 11898..12541 Replicon plasmid pOW1E2a
Accession NZ_CP067246
Organism Escherichia coli strain OW1E2

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag JJB17_RS23225 Protein ID WP_001044768.1
Coordinates 12125..12541 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag JJB17_RS23220 Protein ID WP_001261287.1
Coordinates 11898..12128 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB17_RS23200 (7058) 7058..8146 + 1089 WP_000952217.1 transcriptional repressor PifC -
JJB17_RS23205 (8148) 8148..10373 + 2226 WP_000698737.1 P-loop NTPase fold protein -
JJB17_RS23210 (10423) 10423..11322 - 900 WP_000963206.1 nucleotide-binding protein -
JJB17_RS23215 (11312) 11312..11602 - 291 WP_000111771.1 hypothetical protein -
JJB17_RS23220 (11898) 11898..12128 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
JJB17_RS23225 (12125) 12125..12541 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
JJB17_RS23230 (12703) 12703..14841 - 2139 WP_000350638.1 AAA family ATPase -
JJB17_RS23235 (15195) 15195..15452 + 258 WP_000343085.1 hypothetical protein -
JJB17_RS23240 (15452) 15452..16042 + 591 WP_000194575.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / fosA3 / blaTEM-1B / blaCTX-M-55 - 1..108766 108766


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T185897 WP_001044768.1 NZ_CP067246:12125-12541 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T185897 NZ_CP089052:254749-255051 [Acinetobacter ursingii]
ATGTACTCAATATATACCACTGAAGTCTTTGATGACTGGTTCACCAAGTTAAAAGACCAGCAGGCAAAAAGACGCATACA
AGTCCGAATTGATCGGGTTGAAGATGGAAACTTTGGAGATACTGAGCCGGTCGGTGAAGGTGTTTCTGAATTACGTTTCT
TCTTTGGGCCAGGCTATAGGATTTATTACTGCAAGCAAGGGCAAAGGGTTGTTATTCTTTTAGCAGGCGGAGATAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTGCAATTGGCACAAGATTTAGAGGAGGAGCTATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT185897 WP_001261287.1 NZ_CP067246:11898-12128 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT185897 NZ_CP089052:255052-255333 [Acinetobacter ursingii]
ATGGCTATTAAACTTCGCAAATGGGACAGTGCTGAGCATCTTAAAACAGAGGATGATATGCAGGCTTACTTACAAGCATG
TATTGAAGAATCTAATGGCGATGCCGCATTCATTGCAAAGGCTTTAGGTAACATTGCAAAAGCTAAAGGAATGGCTCAAT
TATCACGAGATACAGGCTTAGGCCGAGAAAGTCTTTATAAAGCACTTTCTGGTGATGTTAACCCTAGTTTTGATACTGTG
ATTAAAGTTGTAAAAGCACTGAATTTACGCTTAGCAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References