Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3952155..3952809 Replicon chromosome
Accession NZ_CP067245
Organism Escherichia coli strain OW1E2

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag JJB17_RS19070 Protein ID WP_000244781.1
Coordinates 3952402..3952809 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JJB17_RS19065 Protein ID WP_000354046.1
Coordinates 3952155..3952421 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB17_RS19040 (3947324) 3947324..3948067 + 744 WP_000951964.1 SDR family oxidoreductase -
JJB17_RS19045 (3948124) 3948124..3949557 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
JJB17_RS19050 (3949602) 3949602..3949913 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
JJB17_RS19055 (3950077) 3950077..3950736 + 660 WP_000250274.1 hemolysin III family protein -
JJB17_RS19060 (3950932) 3950932..3951912 - 981 WP_000886083.1 tRNA-modifying protein YgfZ -
JJB17_RS19065 (3952155) 3952155..3952421 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JJB17_RS19070 (3952402) 3952402..3952809 + 408 WP_000244781.1 protein YgfX Toxin
JJB17_RS19075 (3952849) 3952849..3953370 - 522 WP_001055874.1 flavodoxin FldB -
JJB17_RS19080 (3953482) 3953482..3954378 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
JJB17_RS19085 (3954403) 3954403..3955113 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JJB17_RS19090 (3955119) 3955119..3956852 + 1734 WP_000813201.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T185895 WP_000244781.1 NZ_CP067245:3952402-3952809 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T185895 NZ_CP089052:249414-249710 [Acinetobacter ursingii]
ATGATTGAAATTAAACGTCTTCCAGAATTTGATGAATGGTTGGATGGCATTAAAGACAATATGACCCGTATCCGCCTGAA
TCGTCGATTAGATAAGGTTCAACGTGGAAACTGGGGAGACATTAAACCTCTCCAAGATGGCGTTTGGGAAATGAGAGAAT
TCTTCGGTTCTGGATGGAGGATGTATTACATCCAACATGGTGACGTTGTGATTGTAATGCTTGGTGGTGGAGATAAATCA
ACTCAACAACAAGACATCGATCGGGCAGTCAAACTATCGAAAACATTGGAGGATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT185895 WP_000354046.1 NZ_CP067245:3952155-3952421 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT185895 NZ_CP089052:249712-250002 [Acinetobacter ursingii]
ATGGTTAAAGTCGCTGATTTACCAAGTTTTGATATGGCTGAGTCGCTCAAAACTGAAGAAGACATTGTGATGTATCTCAA
TATGGTTCTTGAAGAAAATGATCCAGCTGAATTAGCCCATGCACTTGGTGTTATTGCTAAAGCTCGTGGCATGACTCAAA
TCGCTAAAGAAGCAGGGATTGGGCGTGAAGCACTCTACAAAGCCCTACGTCAGGATTCAGCACCACGTTTTGATACGATT
AATCGTGTAGTAAATGCTTTAGGCTTAAAGCTCACAGTACAACACGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References