Detailed information of TA system TA185891

Bioinformatically predicted

Overview

TA module

• Type: V
• Classification (family/domain): ghoTS/ghoT-GhoS
• Location: 2607009..2607506
• Replicon: chromosome
• Accession: NZ_CP067245
• Organism: Escherichia coli strain OW1E2

Toxin (Protein)

• Gene name: ghoT
• Uniprot ID: L4JJG6
• Locus tag: JJB17_RS12705
• Protein ID: WP_001173343.1
• Coordinates: 2607009..2607182 (-)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: ghoS
• Uniprot ID: -
• Locus tag: JJB17_RS12710
• Protein ID: WP_072280288.1
• Coordinates: 2607210..2607506 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB17_RS12695 (2603674)	2603674..2605131	+	1458	WP_000856829.1	dipeptide/tripeptide permease DtpC	-
JJB17_RS12700 (2605368)	2605368..2606885	+	1518	WP_001295074.1	lysine--tRNA ligase	-
JJB17_RS12705 (2607009)	2607009..2607182	-	174	WP_001173343.1	type V toxin-antitoxin system toxin GhoT	Toxin
JJB17_RS12710 (2607210)	2607210..2607506	-	297	WP_072280288.1	type V toxin-antitoxin system endoribonuclease antitoxin GhoS	Antitoxin
JJB17_RS12715 (2607733)	2607733..2608005	-	273	WP_000405647.1	GNAT family N-acetyltransferase	-
JJB17_RS12720 (2608017)	2608017..2608247	-	231	WP_000371700.1	4Fe-4S mono-cluster protein YjdI	-
JJB17_RS12725 (2608428)	2608428..2610059	+	1632	WP_113078680.1	sensor histidine kinase	-
JJB17_RS12730 (2610056)	2610056..2610775	+	720	WP_000611288.1	two-component system response regulator DcuR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin GhoT/OrtT (4-57)

Antitoxin

• Endoribonuclease antitoxin GhoS (5-94)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6555.06 Da
• Isoelectric Point: 10.2288

>T185891 WP_001173343.1 NZ_CP067245:c2607182-2607009 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Nucleotide

• Length: 174 bp

>T185891 NZ_CP089048:c31241-30975 [Acinetobacter ursingii]
ATGCAAGTATTTACATATACAGATGCACGTAACAATCTAAAATCCGTTTTAGATAAGGTTATTGATGATGCAGATGTAGC
AGTAATTACTAGGAAAGAAGGTAGTCATGCCGTTGTTATGGGGCAAGAACACTATGACAGCTTAATGGAAACTTTATATC
TTCTTTCATCTCCTAATAATGCTGCTCGTTTAAATGAGTCAATTGCCCAATTACGTGCATCCCGTACTACTGAAAGGGAT
CTCATCGAAGATGAATCGGACAATTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11482.00 Da
• Isoelectric Point: 4.1705

>AT185891 WP_072280288.1 NZ_CP067245:c2607506-2607210 [Escherichia coli]
MEGKNKFNTYIVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Nucleotide

• Length: 297 bp

>AT185891 NZ_CP089048:c30991-30731 [Acinetobacter ursingii]
ATGAATCGGACAATTGAGTTTACAGATAACGCTTGGGATGATTATATCTACTGGCAAACGCAGGATAAAAAGGTACTCAA
GCGTATAAATGCTTTAATCAAAGAGTGCCTACGAACACCATTTGAAGGAACAGGAAAGCCCGAACCACTTAAGGCGAACC
TTTCAGGCTTTTGGAGTCGAAGGATAGATGAAAAGCATAGGTTGGTTTATGAAGTTACGGATGAACGGATTAGCATCGTT
CAGTGTCGGTTTCACTACTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QI41