Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1797108..1797726 Replicon chromosome
Accession NZ_CP067245
Organism Escherichia coli strain OW1E2

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JJB17_RS08730 Protein ID WP_001291435.1
Coordinates 1797508..1797726 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JJB17_RS08725 Protein ID WP_000344800.1
Coordinates 1797108..1797482 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB17_RS08715 (1792197) 1792197..1793390 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JJB17_RS08720 (1793413) 1793413..1796562 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
JJB17_RS08725 (1797108) 1797108..1797482 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JJB17_RS08730 (1797508) 1797508..1797726 + 219 WP_001291435.1 HHA domain-containing protein Toxin
JJB17_RS08735 (1797898) 1797898..1798449 + 552 WP_000102564.1 maltose O-acetyltransferase -
JJB17_RS08740 (1798565) 1798565..1799035 + 471 WP_000136192.1 YlaC family protein -
JJB17_RS08745 (1799199) 1799199..1800749 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JJB17_RS08750 (1800791) 1800791..1801144 - 354 WP_000878140.1 DUF1428 domain-containing protein -
JJB17_RS08760 (1801523) 1801523..1801834 + 312 WP_000409911.1 MGMT family protein -
JJB17_RS08765 (1801865) 1801865..1802437 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T185887 WP_001291435.1 NZ_CP067245:1797508-1797726 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T185887 NZ_CP089044:c1494707-1494426 [Acinetobacter ursingii]
TTGATTAAGAGTTTTAAGCACAAGGGGTTGCAAGCCTTTTTTGAAGATGGCTCAACCGCTGGTATCCAGTCTGCTCACTC
TAAAAAATTACGCTTACTTTTAGCTTCACTAAATTCTGCCACCTCAGTTTATGATTTAAAAACACCACCAAATTGGCGCT
TACATGAACTAAAAGGCAACCTTGAAGGCCACTGGTCTTTAACAGTAAACGGTAATTGGCGTGTAACGTTTAAATTTGAA
GATGGTCATGCATACATCGTGGATTATCAAGACTATCACTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT185887 WP_000344800.1 NZ_CP067245:1797108-1797482 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT185887 NZ_CP089044:c1494396-1494112 [Acinetobacter ursingii]
ATGAAAGTTATGTTTAATGCGCCACACCCTGGTGAAATTCTTCAAGAATATCTTGAAGGGATTTCGGTTACTGACGCAGC
ACGAGCATTGGACGTTACTCGTACAAACTTGTCTCGCATCCTCAATGGTCATACTGGTATATCAGCCGATATGGCGCTTC
GTCTCTCAGAAGCCTTAAGTACTTCACCAGAACTCTGGTTAAACTTACAAGTACAGTATGATCTTTGGATTGCTAGCCAA
ACTAAACGACCTACAATTCAACATTTAACGCCCGCAACGGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References