Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 121152..121920 Replicon chromosome
Accession NZ_CP067245
Organism Escherichia coli strain OW1E2

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag JJB17_RS00620 Protein ID WP_000854814.1
Coordinates 121152..121526 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A3W4SUB1
Locus tag JJB17_RS00625 Protein ID WP_044863316.1
Coordinates 121615..121920 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB17_RS00580 (116548) 116548..117714 + 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
JJB17_RS00585 (117833) 117833..118306 + 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
JJB17_RS00590 (118504) 118504..119562 + 1059 WP_001200891.1 FUSC family protein -
JJB17_RS00595 (119734) 119734..120063 + 330 WP_000450409.1 DUF496 family protein -
JJB17_RS00600 (120164) 120164..120298 - 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
JJB17_RS00605 (120418) 120418..120546 + 129 Protein_120 transposase domain-containing protein -
JJB17_RS00610 (120835) 120835..120915 - 81 Protein_121 hypothetical protein -
JJB17_RS00615 (120961) 120961..121155 - 195 WP_000988600.1 DUF5983 family protein -
JJB17_RS00620 (121152) 121152..121526 - 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
JJB17_RS00625 (121615) 121615..121920 - 306 WP_044863316.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
JJB17_RS00630 (122057) 122057..122278 - 222 WP_000692323.1 DUF987 domain-containing protein -
JJB17_RS00635 (122341) 122341..122817 - 477 WP_001186726.1 RadC family protein -
JJB17_RS00640 (122833) 122833..123312 - 480 WP_032241953.1 antirestriction protein -
JJB17_RS00645 (123394) 123394..124212 - 819 WP_001234629.1 DUF932 domain-containing protein -
JJB17_RS00650 (124312) 124312..124545 - 234 WP_001119729.1 DUF905 family protein -
JJB17_RS00655 (124624) 124624..125079 - 456 WP_208789855.1 IrmA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(2-96)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T185877 WP_000854814.1 NZ_CP067245:c121526-121152 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T185877 NZ_CP089036:c4666081-4665767 [Escherichia coli O157:H7]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCTCCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11250.00 Da        Isoelectric Point: 7.2621

>AT185877 WP_044863316.1 NZ_CP067245:c121920-121615 [Escherichia coli]
MPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKA
DTLSSCGYVYLAVYPTPEMKN

Download         Length: 306 bp

>AT185877 NZ_CP089036:c4666317-4666084 [Escherichia coli O157:H7]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CTGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB A0A3W4SUB1

References