Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 17468..18111 Replicon plasmid pP19_598a
Accession NZ_CP067242
Organism Escherichia coli strain P19_598

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag JJB18_RS23975 Protein ID WP_001044768.1
Coordinates 17695..18111 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag JJB18_RS23970 Protein ID WP_001261287.1
Coordinates 17468..17698 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB18_RS23950 (12628) 12628..13716 + 1089 WP_000952217.1 transcriptional repressor PifC -
JJB18_RS23955 (13718) 13718..15943 + 2226 WP_000698737.1 P-loop NTPase fold protein -
JJB18_RS23960 (15993) 15993..16892 - 900 WP_000963206.1 nucleotide-binding protein -
JJB18_RS23965 (16882) 16882..17172 - 291 WP_000111771.1 hypothetical protein -
JJB18_RS23970 (17468) 17468..17698 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
JJB18_RS23975 (17695) 17695..18111 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
JJB18_RS23980 (18273) 18273..20411 - 2139 WP_000350638.1 AAA family ATPase -
JJB18_RS23985 (20765) 20765..21022 + 258 WP_000343085.1 hypothetical protein -
JJB18_RS23990 (21022) 21022..21612 + 591 WP_000194575.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / fosA3 / blaCTX-M-27 / dfrA14 / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aph(3')-Ia / mph(A) / sitABCD - 1..144175 144175


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T185872 WP_001044768.1 NZ_CP067242:17695-18111 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T185872 NZ_CP089036:c3541497-3541342 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT185872 WP_001261287.1 NZ_CP067242:17468-17698 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT185872 NZ_CP089036:3541509-3541567 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References