Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4524430..4524927 Replicon chromosome
Accession NZ_CP067241
Organism Escherichia coli strain P19_598

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JJB18_RS21435 Protein ID WP_001173343.1
Coordinates 4524430..4524603 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag JJB18_RS21440 Protein ID WP_000398619.1
Coordinates 4524631..4524927 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB18_RS21425 (4521100) 4521100..4522557 + 1458 WP_061092757.1 dipeptide/tripeptide permease DtpC -
JJB18_RS21430 (4522794) 4522794..4524311 + 1518 WP_000003806.1 lysine--tRNA ligase -
JJB18_RS21435 (4524430) 4524430..4524603 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JJB18_RS21440 (4524631) 4524631..4524927 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JJB18_RS21445 (4525156) 4525156..4525428 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
JJB18_RS21450 (4525440) 4525440..4525670 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
JJB18_RS21455 (4525851) 4525851..4527482 + 1632 WP_061092758.1 sensor histidine kinase -
JJB18_RS21460 (4527479) 4527479..4528198 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T185870 WP_001173343.1 NZ_CP067241:c4524603-4524430 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T185870 NZ_CP089036:3097551-3097658 [Escherichia coli O157:H7]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT185870 WP_000398619.1 NZ_CP067241:c4524927-4524631 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT185870 NZ_CP089036:c3097498-3097437 [Escherichia coli O157:H7]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References