Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4343821..4344416 Replicon chromosome
Accession NZ_CP067241
Organism Escherichia coli strain P19_598

Toxin (Protein)


Gene name chpB Uniprot ID V0SXT4
Locus tag JJB18_RS20575 Protein ID WP_000239581.1
Coordinates 4343821..4344171 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID L4JJX7
Locus tag JJB18_RS20580 Protein ID WP_001223213.1
Coordinates 4344165..4344416 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB18_RS20555 (4339275) 4339275..4340297 - 1023 WP_001361374.1 ABC transporter permease -
JJB18_RS20560 (4340311) 4340311..4341813 - 1503 WP_001095661.1 ATP-binding cassette domain-containing protein -
JJB18_RS20565 (4341946) 4341946..4342902 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
JJB18_RS20570 (4343212) 4343212..4343742 + 531 WP_000055072.1 inorganic diphosphatase -
JJB18_RS20575 (4343821) 4343821..4344171 - 351 WP_000239581.1 endoribonuclease toxin ChpB Toxin
JJB18_RS20580 (4344165) 4344165..4344416 - 252 WP_001223213.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
JJB18_RS20585 (4344628) 4344628..4344969 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
JJB18_RS20590 (4344972) 4344972..4348751 - 3780 WP_000060873.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12453.41 Da        Isoelectric Point: 6.2206

>T185868 WP_000239581.1 NZ_CP067241:c4344171-4343821 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Download         Length: 351 bp

>T185868 NZ_CP089036:c2839984-2839808 [Escherichia coli O157:H7]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9331.64 Da        Isoelectric Point: 4.6509

>AT185868 WP_001223213.1 NZ_CP067241:c4344416-4344165 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT185868 NZ_CP089036:c2839762-2839346 [Escherichia coli O157:H7]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SXT4

Antitoxin

Source ID Structure
AlphaFold DB L4JJX7

References