Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4236296..4236872 Replicon chromosome
Accession NZ_CP067241
Organism Escherichia coli strain P19_598

Toxin (Protein)


Gene name shpA Uniprot ID A0A2U2VE01
Locus tag JJB18_RS20090 Protein ID WP_001131965.1
Coordinates 4236585..4236872 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2U2VE39
Locus tag JJB18_RS20085 Protein ID WP_000063158.1
Coordinates 4236296..4236598 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB18_RS20065 (4231404) 4231404..4232624 + 1221 WP_000873756.1 amino acid deaminase -
JJB18_RS20070 (4232892) 4232892..4235042 + 2151 WP_001314407.1 pyruvate/proton symporter BtsT -
JJB18_RS20075 (4235092) 4235092..4235295 + 204 WP_000467859.1 YbdD/YjiX family protein -
JJB18_RS20080 (4235306) 4235306..4236262 + 957 WP_001361361.1 GTPase -
JJB18_RS20085 (4236296) 4236296..4236598 - 303 WP_000063158.1 BrnA antitoxin family protein Antitoxin
JJB18_RS20090 (4236585) 4236585..4236872 - 288 WP_001131965.1 BrnT family toxin Toxin
JJB18_RS20095 (4237069) 4237069..4238235 + 1167 WP_000800823.1 restriction endonuclease -
JJB18_RS20100 (4238280) 4238280..4238453 + 174 Protein_3987 type I restriction-modification system subunit M N-terminal domain-containing protein -
JJB18_RS20105 (4238447) 4238447..4239115 + 669 Protein_3988 DUF3387 domain-containing protein -
JJB18_RS20110 (4239330) 4239330..4239668 + 339 WP_000132633.1 endoribonuclease SymE -
JJB18_RS25090 (4239756) 4239756..4239803 - 48 WP_229017048.1 hypothetical protein -
JJB18_RS20120 (4240051) 4240051..4241463 + 1413 WP_000199309.1 PLP-dependent aminotransferase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11223.71 Da        Isoelectric Point: 7.4021

>T185866 WP_001131965.1 NZ_CP067241:c4236872-4236585 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T185866 NZ_CP089036:c2625542-2625264 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11537.99 Da        Isoelectric Point: 9.4646

>AT185866 WP_000063158.1 NZ_CP067241:c4236598-4236296 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDSDVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT185866 NZ_CP089036:c2625735-2625544 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U2VE01

Antitoxin

Source ID Structure
AlphaFold DB A0A2U2VE39

References