185865

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3740520..3741357 Replicon chromosome
Accession NZ_CP067241
Organism Escherichia coli strain P19_598

Toxin (Protein)


Gene name itaT Uniprot ID A0A1M0D5U3
Locus tag JJB18_RS17725 Protein ID WP_000227782.1
Coordinates 3740815..3741357 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID B1LJI1
Locus tag JJB18_RS17720 Protein ID WP_001353405.1
Coordinates 3740520..3740831 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB18_RS17695 (3735540) 3735540..3736487 + 948 WP_001239437.1 cytochrome o ubiquinol oxidase subunit II -
JJB18_RS17700 (3736509) 3736509..3738500 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
JJB18_RS17705 (3738490) 3738490..3739104 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
JJB18_RS17710 (3739104) 3739104..3739433 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
JJB18_RS17715 (3739445) 3739445..3740335 + 891 WP_000971327.1 heme o synthase -
JJB18_RS17720 (3740520) 3740520..3740831 + 312 WP_001353405.1 DUF1778 domain-containing protein Antitoxin
JJB18_RS17725 (3740815) 3740815..3741357 + 543 WP_000227782.1 GNAT family N-acetyltransferase Toxin
JJB18_RS17730 (3741413) 3741413..3742257 - 845 Protein_3519 tetratricopeptide repeat protein -
JJB18_RS17735 (3742664) 3742664..3744028 + 1365 WP_001000978.1 MFS transporter -
JJB18_RS17740 (3744156) 3744156..3744647 - 492 WP_001138904.1 nucleotide binding protein YajQ -
JJB18_RS17745 (3744815) 3744815..3745726 + 912 WP_000705841.1 2-dehydropantoate 2-reductase -
JJB18_RS17750 (3745689) 3745689..3746279 + 591 WP_001276320.1 protein deglycase YajL -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(19-96)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19957.31 Da        Isoelectric Point: 9.1763

>T185865 WP_000227782.1 NZ_CP067241:3740815-3741357 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDIIYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCNFFEHVKIIHRALPIKGVYLDADPAAINFYTRLGFVQLSA
RPNVFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T185865 NZ_CP089036:2348299-2348402 [Escherichia coli O157:H7]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11370.09 Da        Isoelectric Point: 10.4825

>AT185865 WP_001353405.1 NZ_CP067241:3740520-3740831 [Escherichia coli]
MPGKTVALPDIDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT185865 NZ_CP089036:c2348440-2348169 [Escherichia coli O157:H7]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1M0D5U3

Antitoxin

Source ID Structure
AlphaFold DB A0A829G7G3

References