Detailed information of TA system TA185862

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3280009..3280714
• Replicon: chromosome
• Accession: NZ_CP067241
• Organism: Escherichia coli strain P19_598

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A1M2J6W7
• Locus tag: JJB18_RS15425
• Protein ID: WP_000539524.1
• Coordinates: 3280009..3280395 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: JJB18_RS15430
• Protein ID: WP_001280945.1
• Coordinates: 3280385..3280714 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB18_RS15405 (3276013)	3276013..3276639	+	627	WP_001295292.1	glutathione S-transferase GstB	-
JJB18_RS15410 (3276636)	3276636..3277751	-	1116	WP_000555056.1	aldose sugar dehydrogenase YliI	-
JJB18_RS15415 (3277862)	3277862..3278245	-	384	WP_000497137.1	biofilm formation regulator BssR	-
JJB18_RS15420 (3278458)	3278458..3279783	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
JJB18_RS15425 (3280009)	3280009..3280395	+	387	WP_000539524.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JJB18_RS15430 (3280385)	3280385..3280714	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
JJB18_RS15435 (3280784)	3280784..3282101	-	1318	Protein_3071	diguanylate cyclase	-
JJB18_RS15440 (3282109)	3282109..3284457	-	2349	WP_000950331.1	EAL domain-containing protein	-
JJB18_RS15445 (3284634)	3284634..3285545	-	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14321.46 Da
• Isoelectric Point: 9.9521

>T185862 WP_000539524.1 NZ_CP067241:3280009-3280395 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSRSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T185862 NZ_CP089036:745386-745700 [Escherichia coli O157:H7]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTAGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTTTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT185862 WP_001280945.1 NZ_CP067241:3280385-3280714 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT185862 NZ_CP089036:745697-746113 [Escherichia coli O157:H7]
ATGATTGCGATTGCCGACATCTTGCATGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACATCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: A0A1M2J6W7