Detailed information of TA system TA185852

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2098001..2098785
• Replicon: chromosome
• Accession: NZ_CP067241
• Organism: Escherichia coli strain P19_598

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: JJB18_RS09720
• Protein ID: WP_000613626.1
• Coordinates: 2098001..2098495 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: JJB18_RS09725
• Protein ID: WP_001110447.1
• Coordinates: 2098492..2098785 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB18_RS09700 (2093194)	2093194..2094291	+	1098	WP_000589326.1	flagellar basal body P-ring protein FlgI	-
JJB18_RS09705 (2094291)	2094291..2095232	+	942	WP_001305885.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
JJB18_RS09710 (2095298)	2095298..2096941	+	1644	WP_061092652.1	flagellar hook-associated protein FlgK	-
JJB18_RS09715 (2096953)	2096953..2097906	+	954	WP_001212762.1	flagellar hook-associated protein FlgL	-
JJB18_RS09720 (2098001)	2098001..2098495	-	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
JJB18_RS09725 (2098492)	2098492..2098785	-	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
JJB18_RS09730 (2098918)	2098918..2102103	-	3186	WP_061092653.1	ribonuclease E	-
JJB18_RS09735 (2102676)	2102676..2103635	+	960	WP_000846342.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T185852 WP_000613626.1 NZ_CP067241:c2098495-2098001 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T185852 NZ_CP089034:4965290-4965631 [Escherichia coli O157:H7]
ATGACTGATACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGTTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTTGCCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT185852 WP_001110447.1 NZ_CP067241:c2098785-2098492 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT185852 NZ_CP089034:c4965295-4965219 [Escherichia coli O157:H7]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6