Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1088260..1088914 Replicon chromosome
Accession NZ_CP067241
Organism Escherichia coli strain P19_598

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag JJB18_RS05190 Protein ID WP_000244781.1
Coordinates 1088507..1088914 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JJB18_RS05185 Protein ID WP_000354046.1
Coordinates 1088260..1088526 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB18_RS05165 (1084348) 1084348..1085781 - 1434 WP_001521235.1 6-phospho-beta-glucosidase BglA -
JJB18_RS05170 (1085826) 1085826..1086137 + 312 WP_001182949.1 N(4)-acetylcytidine aminohydrolase -
JJB18_RS05175 (1086301) 1086301..1086960 + 660 WP_000250269.1 hemolysin III family protein -
JJB18_RS05180 (1087037) 1087037..1088017 - 981 WP_000886050.1 tRNA-modifying protein YgfZ -
JJB18_RS05185 (1088260) 1088260..1088526 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JJB18_RS05190 (1088507) 1088507..1088914 + 408 WP_000244781.1 protein YgfX Toxin
JJB18_RS05195 (1088954) 1088954..1089475 - 522 WP_001521233.1 flavodoxin FldB -
JJB18_RS05200 (1089587) 1089587..1090483 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
JJB18_RS05205 (1090508) 1090508..1091218 + 711 WP_001521231.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JJB18_RS05210 (1091224) 1091224..1092957 + 1734 WP_063117505.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T185850 WP_000244781.1 NZ_CP067241:1088507-1088914 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T185850 NZ_CP089034:c4614265-4613867 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT185850 WP_000354046.1 NZ_CP067241:1088260-1088526 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT185850 NZ_CP089034:c4614561-4614268 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References