Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 18881..19403 Replicon plasmid pSBF22c
Accession NZ_CP067238
Organism Escherichia coli strain SBF22

Toxin (Protein)


Gene name relE Uniprot ID D0QMR1
Locus tag JJB19_RS25245 Protein ID WP_000220561.1
Coordinates 19122..19403 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag JJB19_RS25240 Protein ID WP_000121743.1
Coordinates 18881..19132 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB19_RS25215 (14120) 14120..14980 + 861 WP_063122221.1 hypothetical protein -
JJB19_RS25220 (15001) 15001..15357 - 357 WP_001714234.1 DNA distortion polypeptide 3 -
JJB19_RS25225 (15494) 15494..15940 - 447 WP_001074384.1 hypothetical protein -
JJB19_RS25230 (15944) 15944..16786 - 843 WP_000435064.1 replication initiation protein -
JJB19_RS25235 (16807) 16807..17646 - 840 WP_001352874.1 replication initiation protein -
JJB19_RS25240 (18881) 18881..19132 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
JJB19_RS25245 (19122) 19122..19403 + 282 WP_000220561.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JJB19_RS25250 (19753) 19753..20268 + 516 WP_001025387.1 J domain-containing protein -
JJB19_RS25255 (20342) 20342..20890 + 549 WP_001178641.1 hypothetical protein -
JJB19_RS25260 (20899) 20899..21168 + 270 WP_001006718.1 hypothetical protein -
JJB19_RS25265 (21158) 21158..21400 + 243 WP_000008708.1 hypothetical protein -
JJB19_RS25270 (21533) 21533..22237 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
JJB19_RS25275 (22243) 22243..22383 + 141 WP_001044210.1 hypothetical protein -
JJB19_RS25280 (22869) 22869..23606 + 738 WP_001446887.1 recombinase family protein -
JJB19_RS25285 (23603) 23603..23827 + 225 WP_000743213.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..25897 25897
- flank IS/Tn - - 21533..22237 704


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11023.86 Da        Isoelectric Point: 10.5938

>T185842 WP_000220561.1 NZ_CP067238:19122-19403 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Download         Length: 282 bp

>T185842 NZ_CP089034:c2542996-2542718 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT185842 WP_000121743.1 NZ_CP067238:18881-19132 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT185842 NZ_CP089034:c2543189-2542998 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S5I302

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References