Detailed information of TA system TA185841

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 27420..28021
• Replicon: plasmid pSBF22b
• Accession: NZ_CP067237
• Organism: Escherichia coli strain SBF22

Toxin (Protein)

• Gene name: doc
• Uniprot ID: U9YA20
• Locus tag: JJB19_RS24685
• Protein ID: WP_001216045.1
• Coordinates: 27420..27800 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: JJB19_RS24690
• Protein ID: WP_001190712.1
• Coordinates: 27800..28021 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB19_RS24660 (JJB19_24655)	22860..24302	-	1443	WP_001472843.1	hypothetical protein	-
JJB19_RS24665 (JJB19_24660)	24344..25537	-	1194	WP_106489616.1	terminase	-
JJB19_RS24670 (JJB19_24665)	25624..26076	-	453	WP_001326849.1	late promoter-activating protein	-
JJB19_RS24675 (JJB19_24670)	26165..27208	-	1044	WP_106489615.1	DUF968 domain-containing protein	-
JJB19_RS24680 (JJB19_24675)	27236..27415	-	180	WP_001339207.1	hypothetical protein	-
JJB19_RS24685 (JJB19_24680)	27420..27800	-	381	WP_001216045.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
JJB19_RS24690 (JJB19_24685)	27800..28021	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JJB19_RS24695 (JJB19_24690)	28094..28483	-	390	WP_000506730.1	S24 family peptidase	-
JJB19_RS24700 (JJB19_24695)	28658..29242	+	585	WP_044068914.1	hypothetical protein	-
JJB19_RS24705 (JJB19_24700)	29243..29605	+	363	WP_044068912.1	hypothetical protein	-
JJB19_RS24710 (JJB19_24705)	29617..29856	-	240	WP_023356286.1	DNA polymerase III subunit theta	-
JJB19_RS24715 (JJB19_24710)	30171..30944	-	774	WP_223677252.1	hypothetical protein	-
JJB19_RS24720 (JJB19_24715)	30926..31300	-	375	WP_032353387.1	hypothetical protein	-
JJB19_RS24725 (JJB19_24720)	31307..31600	-	294	WP_000269001.1	hypothetical protein	-
JJB19_RS24730 (JJB19_24725)	31779..32012	-	234	WP_000517421.1	hypothetical protein	-
JJB19_RS24735 (JJB19_24730)	32095..32781	-	687	WP_234287463.1	DUF550 domain-containing protein	-
JJB19_RS25740	32755..32880	-	126	Protein_35	DUF550 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..96901	96901

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13588.29 Da
• Isoelectric Point: 5.1514

>T185841 WP_001216045.1 NZ_CP067237:c27800-27420 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T185841 NZ_CP089034:2265688-2265791 [Escherichia coli O157:H7]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT185841 WP_001190712.1 NZ_CP067237:c28021-27800 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT185841 NZ_CP089034:c2265829-2265558 [Escherichia coli O157:H7]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: A0A829CLP7

Antitoxin

• AlphaFold DB: A0A829CJB6