Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 17053..17578 Replicon plasmid pSBF22a
Accession NZ_CP067236
Organism Escherichia coli strain SBF22

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag JJB19_RS23955 Protein ID WP_001159871.1
Coordinates 17053..17358 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag JJB19_RS23960 Protein ID WP_000813634.1
Coordinates 17360..17578 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB19_RS23935 (13548) 13548..14918 + 1371 WP_001312828.1 efflux transporter outer membrane subunit -
JJB19_RS23940 (15392) 15392..15583 + 192 Protein_13 IS200/IS605-like element IS200C family transposase -
JJB19_RS23945 (15670) 15670..16374 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
JJB19_RS23950 (16438) 16438..17052 - 615 Protein_15 tyrosine-type recombinase/integrase -
JJB19_RS23955 (17053) 17053..17358 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
JJB19_RS23960 (17360) 17360..17578 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
JJB19_RS23965 (18045) 18045..18740 + 696 WP_072652234.1 DNA-binding protein -
JJB19_RS23970 (18737) 18737..19357 + 621 WP_072652229.1 hypothetical protein -
JJB19_RS25690 (19354) 19354..19545 + 192 Protein_20 hypothetical protein -
JJB19_RS23975 (19561) 19561..20142 + 582 WP_228719449.1 DUF2971 domain-containing protein -
JJB19_RS23980 (20178) 20178..20918 + 741 WP_000933099.1 abortive infection family protein -
JJB19_RS23985 (21077) 21077..21706 - 630 Protein_23 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(A) / blaTEM-1B / tet(B) / catA1 / blaCTX-M-65 / fosA3 / sul2 / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / sitABCD afaC-I / iutA / iucD / iucC / iucB / iucA 1..122982 122982


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T185832 WP_001159871.1 NZ_CP067236:c17358-17053 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T185832 NZ_CP089033:49099-49404 [Escherichia coli O157:H7]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT185832 WP_000813634.1 NZ_CP067236:c17578-17360 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT185832 NZ_CP089033:48879-49097 [Escherichia coli O157:H7]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References