Detailed information of TA system TA185831

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 4863492..4864197
• Replicon: chromosome
• Accession: NZ_CP067235
• Organism: Escherichia coli strain SBF22

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: JJB19_RS23365
• Protein ID: WP_000539521.1
• Coordinates: 4863811..4864197 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: JJB19_RS23360
• Protein ID: WP_001280945.1
• Coordinates: 4863492..4863821 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB19_RS23345 (4858650)	4858650..4859561	+	912	WP_001236058.1	glutathione ABC transporter permease GsiD	-
JJB19_RS23350 (4859738)	4859738..4862086	+	2349	WP_000950337.1	EAL domain-containing protein	-
JJB19_RS23355 (4862094)	4862094..4863422	+	1329	WP_000086869.1	GGDEF domain-containing protein	-
JJB19_RS23360 (4863492)	4863492..4863821	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
JJB19_RS23365 (4863811)	4863811..4864197	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JJB19_RS23370 (4864423)	4864423..4865748	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
JJB19_RS23375 (4865961)	4865961..4866344	+	384	WP_000497137.1	biofilm formation regulator BssR	-
JJB19_RS23380 (4866455)	4866455..4867570	+	1116	WP_000555050.1	aldose sugar dehydrogenase YliI	-
JJB19_RS23385 (4867567)	4867567..4868193	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T185831 WP_000539521.1 NZ_CP067235:c4864197-4863811 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T185831 NZ_CP089033:2053-2328 [Escherichia coli O157:H7]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT185831 WP_001280945.1 NZ_CP067235:c4863821-4863492 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT185831 NZ_CP089033:1769-2053 [Escherichia coli O157:H7]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6