Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4508767..4509385 Replicon chromosome
Accession NZ_CP067235
Organism Escherichia coli strain SBF22

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JJB19_RS21670 Protein ID WP_001291435.1
Coordinates 4508767..4508985 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JJB19_RS21675 Protein ID WP_000344800.1
Coordinates 4509011..4509385 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB19_RS21635 (4504057) 4504057..4504629 + 573 WP_000779831.1 YbaY family lipoprotein -
JJB19_RS21640 (4504660) 4504660..4504971 - 312 WP_000409911.1 MGMT family protein -
JJB19_RS21650 (4505350) 4505350..4505703 + 354 WP_000878140.1 DUF1428 domain-containing protein -
JJB19_RS21655 (4505744) 4505744..4507294 - 1551 WP_012311818.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JJB19_RS21660 (4507458) 4507458..4507928 - 471 WP_000136192.1 YlaC family protein -
JJB19_RS21665 (4508044) 4508044..4508595 - 552 WP_000102561.1 maltose O-acetyltransferase -
JJB19_RS21670 (4508767) 4508767..4508985 - 219 WP_001291435.1 HHA domain-containing protein Toxin
JJB19_RS21675 (4509011) 4509011..4509385 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JJB19_RS21680 (4509931) 4509931..4513080 - 3150 WP_072754545.1 efflux RND transporter permease AcrB -
JJB19_RS21685 (4513103) 4513103..4514296 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T185830 WP_001291435.1 NZ_CP067235:c4508985-4508767 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T185830 NZ_CP089032:c5200134-5199961 [Escherichia coli O157:H7]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT185830 WP_000344800.1 NZ_CP067235:c4509385-4509011 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT185830 NZ_CP089032:c5200458-5200162 [Escherichia coli O157:H7]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References