185829

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 4474459..4475296 Replicon chromosome
Accession NZ_CP067235
Organism Escherichia coli strain SBF22

Toxin (Protein)


Gene name itaT Uniprot ID V0SED1
Locus tag JJB19_RS21500 Protein ID WP_000227787.1
Coordinates 4474459..4475001 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID B1LJI1
Locus tag JJB19_RS21505 Protein ID WP_001353405.1
Coordinates 4474985..4475296 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB19_RS21480 (4469999) 4469999..4470910 - 912 WP_000705871.1 2-dehydropantoate 2-reductase -
JJB19_RS21485 (4471078) 4471078..4471569 + 492 WP_001138904.1 nucleotide binding protein YajQ -
JJB19_RS21490 (4471697) 4471697..4473061 - 1365 WP_001000978.1 MFS transporter -
JJB19_RS21495 (4473468) 4473468..4474403 + 936 WP_012311701.1 tetratricopeptide repeat protein -
JJB19_RS21500 (4474459) 4474459..4475001 - 543 WP_000227787.1 GNAT family N-acetyltransferase Toxin
JJB19_RS21505 (4474985) 4474985..4475296 - 312 WP_001353405.1 DUF1778 domain-containing protein Antitoxin
JJB19_RS21510 (4475481) 4475481..4476371 - 891 WP_000971336.1 heme o synthase -
JJB19_RS21515 (4476383) 4476383..4476712 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
JJB19_RS21520 (4476712) 4476712..4477326 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
JJB19_RS21525 (4477316) 4477316..4479307 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
JJB19_RS21530 (4479329) 4479329..4480276 - 948 WP_001239436.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(19-96)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19946.24 Da        Isoelectric Point: 8.8951

>T185829 WP_000227787.1 NZ_CP067235:c4475001-4474459 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHRALPIKGVYLDADPAAINFYTRLGFVQLSA
RPNVFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T185829 NZ_CP089032:c5103320-5102970 [Escherichia coli O157:H7]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11370.09 Da        Isoelectric Point: 10.4825

>AT185829 WP_001353405.1 NZ_CP067235:c4475296-4474985 [Escherichia coli]
MPGKTVALPDIDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT185829 NZ_CP089032:c5103565-5103314 [Escherichia coli O157:H7]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GE43

Antitoxin

Source ID Structure
AlphaFold DB A0A829G7G3

References