Detailed information of TA system TA185827

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 3735789..3736384
• Replicon: chromosome
• Accession: NZ_CP067235
• Organism: Escherichia coli strain SBF22

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: JJB19_RS17740
• Protein ID: WP_000239581.1
• Coordinates: 3736034..3736384 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: JJB19_RS17735
• Protein ID: WP_001223213.1
• Coordinates: 3735789..3736040 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB19_RS17725 (3731454)	3731454..3735233	+	3780	WP_000060872.1	autotransporter assembly complex protein TamB	-
JJB19_RS17730 (3735236)	3735236..3735577	+	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
JJB19_RS17735 (3735789)	3735789..3736040	+	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
JJB19_RS17740 (3736034)	3736034..3736384	+	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
JJB19_RS17745 (3736463)	3736463..3736993	-	531	WP_000055075.1	inorganic diphosphatase	-
JJB19_RS17750 (3737303)	3737303..3738259	+	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
JJB19_RS17755 (3738392)	3738392..3739894	+	1503	WP_001095664.1	sugar ABC transporter ATP-binding protein	-
JJB19_RS17760 (3739908)	3739908..3740930	+	1023	WP_012311695.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T185827 WP_000239581.1 NZ_CP067235:3736034-3736384 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T185827 NZ_CP089032:c4854105-4853791 [Escherichia coli O157:H7]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT185827 WP_001223213.1 NZ_CP067235:3735789-3736040 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT185827 NZ_CP089032:c4854341-4854108 [Escherichia coli O157:H7]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CTGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7