Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2137601..2138255 Replicon chromosome
Accession NZ_CP067235
Organism Escherichia coli strain SBF22

Toxin (Protein)


Gene name cptA Uniprot ID F4T2L4
Locus tag JJB19_RS10200 Protein ID WP_000244765.1
Coordinates 2137601..2138008 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JJB19_RS10205 Protein ID WP_000354046.1
Coordinates 2137989..2138255 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB19_RS10180 (2133558) 2133558..2135291 - 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -
JJB19_RS10185 (2135297) 2135297..2136007 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JJB19_RS10190 (2136032) 2136032..2136928 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
JJB19_RS10195 (2137040) 2137040..2137561 + 522 WP_001055874.1 flavodoxin FldB -
JJB19_RS10200 (2137601) 2137601..2138008 - 408 WP_000244765.1 protein YgfX Toxin
JJB19_RS10205 (2137989) 2137989..2138255 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JJB19_RS10210 (2138498) 2138498..2139478 + 981 WP_049038218.1 tRNA-modifying protein YgfZ -
JJB19_RS10215 (2139555) 2139555..2140214 - 660 WP_000250274.1 hemolysin III family protein -
JJB19_RS10220 (2140378) 2140378..2140689 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
JJB19_RS10225 (2140734) 2140734..2142167 + 1434 WP_049039059.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16043.95 Da        Isoelectric Point: 11.5202

>T185823 WP_000244765.1 NZ_CP067235:c2138008-2137601 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T185823 NZ_CP089032:3692894-3693181 [Escherichia coli O157:H7]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT185823 WP_000354046.1 NZ_CP067235:c2138255-2137989 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT185823 NZ_CP089032:3692703-3692894 [Escherichia coli O157:H7]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A454A7D7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References