Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1103225..1104009 Replicon chromosome
Accession NZ_CP067235
Organism Escherichia coli strain SBF22

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag JJB19_RS05435 Protein ID WP_000613626.1
Coordinates 1103515..1104009 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID B1LI61
Locus tag JJB19_RS05430 Protein ID WP_001110446.1
Coordinates 1103225..1103518 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB19_RS05420 (1098386) 1098386..1099345 - 960 WP_000846337.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -
JJB19_RS05425 (1099918) 1099918..1103091 + 3174 WP_023141578.1 ribonuclease E -
JJB19_RS05430 (1103225) 1103225..1103518 + 294 WP_001110446.1 DUF1778 domain-containing protein Antitoxin
JJB19_RS05435 (1103515) 1103515..1104009 + 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
JJB19_RS05440 (1104104) 1104104..1105057 - 954 WP_001212763.1 flagellar hook-associated protein FlgL -
JJB19_RS05445 (1105069) 1105069..1106712 - 1644 WP_000096448.1 flagellar hook-associated protein FlgK -
JJB19_RS05450 (1106778) 1106778..1107719 - 942 WP_012311956.1 flagellar assembly peptidoglycan hydrolase FlgJ -
JJB19_RS05455 (1107719) 1107719..1108816 - 1098 WP_001441925.1 flagellar basal body P-ring protein FlgI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T185821 WP_000613626.1 NZ_CP067235:1103515-1104009 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

>T185821 NZ_CP089032:3144094-3144201 [Escherichia coli O157:H7]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11079.67 Da        Isoelectric Point: 10.5423

>AT185821 WP_001110446.1 NZ_CP067235:1103225-1103518 [Escherichia coli]
MPAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp

>AT185821 NZ_CP089032:c3144041-3143980 [Escherichia coli O157:H7]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G775

References