Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 780639..781277 Replicon chromosome
Accession NZ_CP067235
Organism Escherichia coli strain SBF22

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag JJB19_RS03885 Protein ID WP_000813794.1
Coordinates 781101..781277 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag JJB19_RS03880 Protein ID WP_001270286.1
Coordinates 780639..781055 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB19_RS03860 (775791) 775791..776732 - 942 WP_001251303.1 ABC transporter permease -
JJB19_RS03865 (776733) 776733..777746 - 1014 WP_000220391.1 ABC transporter ATP-binding protein -
JJB19_RS03870 (777764) 777764..778909 - 1146 WP_000034364.1 ABC transporter substrate-binding protein -
JJB19_RS03875 (779154) 779154..780560 - 1407 WP_000760611.1 PLP-dependent aminotransferase family protein -
JJB19_RS03880 (780639) 780639..781055 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
JJB19_RS03885 (781101) 781101..781277 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
JJB19_RS03890 (781499) 781499..781729 + 231 WP_000491567.1 DUF2554 family protein -
JJB19_RS03895 (781821) 781821..783782 - 1962 WP_012311648.1 23S rRNA 5-hydroxycytidine C2501 synthase -
JJB19_RS03900 (783855) 783855..784391 - 537 WP_000429154.1 DNA-binding transcriptional regulator SutR -
JJB19_RS03905 (784483) 784483..785364 + 882 Protein_777 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 785379..786707 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T185820 WP_000813794.1 NZ_CP067235:c781277-781101 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T185820 NZ_CP089032:3143558-3143665 [Escherichia coli O157:H7]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT185820 WP_001270286.1 NZ_CP067235:c781055-780639 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT185820 NZ_CP089032:c3143510-3143451 [Escherichia coli O157:H7]
GTCTGGTTTCAAGATTAGCCCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References