Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 21798..22441 Replicon plasmid pSBF45a
Accession NZ_CP067233
Organism Escherichia coli strain SBF45

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag JJB20_RS22085 Protein ID WP_001044768.1
Coordinates 21798..22214 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag JJB20_RS22090 Protein ID WP_001261287.1
Coordinates 22211..22441 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB20_RS22070 (18297) 18297..18887 - 591 WP_000194575.1 hypothetical protein -
JJB20_RS22075 (18887) 18887..19144 - 258 WP_000343085.1 hypothetical protein -
JJB20_RS22080 (19498) 19498..21636 + 2139 WP_000350638.1 AAA family ATPase -
JJB20_RS22085 (21798) 21798..22214 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
JJB20_RS22090 (22211) 22211..22441 - 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
JJB20_RS22095 (22737) 22737..23027 + 291 WP_000111771.1 hypothetical protein -
JJB20_RS22100 (23017) 23017..23916 + 900 WP_000963206.1 nucleotide-binding protein -
JJB20_RS22105 (23966) 23966..26191 - 2226 WP_000698737.1 P-loop NTPase fold protein -
JJB20_RS22110 (26193) 26193..27281 - 1089 WP_000952217.1 transcriptional repressor PifC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid fosA3 - 1..107006 107006


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T185806 WP_001044768.1 NZ_CP067233:c22214-21798 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T185806 NZ_CP089031:4705476-4705817 [Escherichia coli]
ATGACTGATACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGTTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTTGCCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT185806 WP_001261287.1 NZ_CP067233:c22441-22211 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT185806 NZ_CP089031:c4705481-4705405 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References