Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 1955242..1955873 Replicon chromosome
Accession NZ_CP067232
Organism Escherichia coli strain SBF45

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag JJB20_RS09100 Protein ID WP_001259388.1
Coordinates 1955242..1955517 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag JJB20_RS09105 Protein ID WP_000593555.1
Coordinates 1955514..1955873 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB20_RS09085 (1950246) 1950246..1951313 + 1068 WP_000361485.1 HlyD family secretion protein -
JJB20_RS09090 (1951310) 1951310..1954045 + 2736 WP_000149125.1 ribosome-associated ATPase/putative transporter RbbA -
JJB20_RS09095 (1954045) 1954045..1955169 + 1125 WP_001314210.1 ABC transporter permease -
JJB20_RS09100 (1955242) 1955242..1955517 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
JJB20_RS09105 (1955514) 1955514..1955873 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
JJB20_RS09110 (1955993) 1955993..1956394 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
JJB20_RS09115 (1956400) 1956400..1957206 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
JJB20_RS09120 (1957203) 1957203..1957967 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
JJB20_RS09125 (1957967) 1957967..1958800 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
JJB20_RS09130 (1958797) 1958797..1959741 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T185789 WP_001259388.1 NZ_CP067232:1955242-1955517 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T185789 NZ_CP089031:c702898-702434 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT185789 WP_000593555.1 NZ_CP067232:1955514-1955873 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT185789 NZ_CP089031:c703233-702898 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAGCAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATACAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References