Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 1083091..1083686 Replicon chromosome
Accession NZ_CP067232
Organism Escherichia coli strain SBF45

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag JJB20_RS05150 Protein ID WP_000239579.1
Coordinates 1083091..1083441 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag JJB20_RS05155 Protein ID WP_001223208.1
Coordinates 1083435..1083686 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB20_RS05130 (1078537) 1078537..1079559 - 1023 WP_024251350.1 ABC transporter permease -
JJB20_RS05135 (1079573) 1079573..1081075 - 1503 WP_000205810.1 sugar ABC transporter ATP-binding protein -
JJB20_RS05140 (1081215) 1081215..1082171 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
JJB20_RS05145 (1082481) 1082481..1083011 + 531 WP_000055075.1 inorganic diphosphatase -
JJB20_RS05150 (1083091) 1083091..1083441 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
JJB20_RS05155 (1083435) 1083435..1083686 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
JJB20_RS05160 (1083898) 1083898..1084239 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
JJB20_RS05165 (1084242) 1084242..1088021 - 3780 WP_000060896.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T185786 WP_000239579.1 NZ_CP067232:c1083441-1083091 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T185786 NZ_CP089031:359393-359668 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT185786 WP_001223208.1 NZ_CP067232:c1083686-1083435 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT185786 NZ_CP089031:359665-360024 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References