Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 397926..398544 Replicon chromosome
Accession NZ_CP067232
Organism Escherichia coli strain SBF45

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JJB20_RS01930 Protein ID WP_001291435.1
Coordinates 398326..398544 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JJB20_RS01925 Protein ID WP_000344800.1
Coordinates 397926..398300 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB20_RS01915 (393015) 393015..394208 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JJB20_RS01920 (394231) 394231..397380 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
JJB20_RS01925 (397926) 397926..398300 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JJB20_RS01930 (398326) 398326..398544 + 219 WP_001291435.1 HHA domain-containing protein Toxin
JJB20_RS01935 (398715) 398715..399266 + 552 WP_000102564.1 maltose O-acetyltransferase -
JJB20_RS01940 (399382) 399382..399852 + 471 WP_000136192.1 YlaC family protein -
JJB20_RS01945 (400016) 400016..401566 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JJB20_RS01950 (401608) 401608..401961 - 354 WP_000878140.1 DUF1428 domain-containing protein -
JJB20_RS01955 (402305) 402305..402532 - 228 WP_024183738.1 DUF2188 domain-containing protein -
JJB20_RS01960 (402749) 402749..403369 - 621 WP_032181884.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T185784 WP_001291435.1 NZ_CP067232:398326-398544 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T185784 NZ_CP089030:c156729-156286 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT185784 WP_000344800.1 NZ_CP067232:397926-398300 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT185784 NZ_CP089030:c156956-156726 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References