Detailed information of TA system TA185783

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 3785..4320
• Replicon: plasmid unnamed3
• Accession: NZ_CP067228
• Organism: Paracoccus sp. MC1862

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: JGR78_RS17220
• Protein ID: WP_182805159.1
• Coordinates: 4072..4320 (-)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: JGR78_RS17215
• Protein ID: WP_182805205.1
• Coordinates: 3785..4036 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JGR78_RS17190	326..1378	+	1053	WP_182805150.1	replication initiator protein A	-
JGR78_RS17195	1385..1735	-	351	WP_182805152.1	stability/partitioning determinant	-
JGR78_RS17200	1737..2423	-	687	WP_182805154.1	ParA family protein	-
JGR78_RS17205	2969..3392	-	424	Protein_3	type II toxin-antitoxin system VapC family toxin	-
JGR78_RS17210	3392..3646	-	255	WP_182805157.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
JGR78_RS17215	3785..4036	-	252	WP_182805205.1	BrnA antitoxin family protein	Antitoxin
JGR78_RS17220	4072..4320	-	249	WP_182805159.1	BrnT family toxin	Toxin
JGR78_RS17225	4354..5142	-	789	WP_200559551.1	hypothetical protein	-
JGR78_RS17230	5736..5996	-	261	WP_182805164.1	type II toxin-antitoxin system PemK/MazF family toxin	-
JGR78_RS17235	5883..6260	+	378	WP_182805207.1	sugar transferase	-
JGR78_RS17240	7277..7636	-	360	WP_182805166.1	type II toxin-antitoxin system VapC family toxin	-
JGR78_RS17245	7633..7857	-	225	WP_182805169.1	hypothetical protein	-
JGR78_RS17250	8345..9187	+	843	WP_182805171.1	replication initiation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..53553	53553

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (7-81)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 8917.33 Da
• Isoelectric Point: 5.0022

>T185783 WP_182805159.1 NZ_CP067228:c4320-4072 [Paracoccus sp. MC1862]
MLMIVWDEPKRQTNLAKHGLDFADLDEGFFLASLVVPAKDGRNMAIGRLADGTIAVVFATLGTEGVSVISMRPASERERS
LL

Nucleotide

• Length: 249 bp

>T185783 NZ_CP089030:c104113-103643 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 8803.93 Da
• Isoelectric Point: 6.2815

>AT185783 WP_182805205.1 NZ_CP067228:c4036-3785 [Paracoccus sp. MC1862]
MIASDPDAPEASDDQLAQAKPFTEAFPALAEAMRKNVGGRPKAENPKVAVSLRLDQDVVARFKASGPGWQTRINSALRDA
AGL

Nucleotide

• Length: 252 bp

>AT185783 NZ_CP089030:c104553-104110 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA