Detailed information of TA system TA185780

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 3992..4527
• Replicon: plasmid p9146
• Accession: NZ_CP067174
• Organism: Paracoccus aestuarii strain DSM 19484

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: JHW48_RS18410
• Protein ID: WP_119887537.1
• Coordinates: 4279..4527 (-)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: JHW48_RS18405
• Protein ID: WP_119887538.1
• Coordinates: 3992..4282 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JHW48_RS18385 (JHW48_18275)	1..9146	-	9146	WP_119887542.1	HAD family hydrolase	-
JHW48_RS18390 (JHW48_18280)	406..1404	-	999	WP_119887541.1	UbiA family prenyltransferase	-
JHW48_RS18395 (JHW48_18285)	1401..2804	-	1404	WP_147388141.1	hypothetical protein	-
JHW48_RS18400 (JHW48_18290)	3242..3883	-	642	WP_272835915.1	hypothetical protein	-
JHW48_RS18405 (JHW48_18295)	3992..4282	-	291	WP_119887538.1	BrnA antitoxin family protein	Antitoxin
JHW48_RS18410 (JHW48_18300)	4279..4527	-	249	WP_119887537.1	BrnT family toxin	Toxin
JHW48_RS18415 (JHW48_18305)	5026..5577	-	552	WP_119887535.1	helix-turn-helix domain-containing protein	-
JHW48_RS18420 (JHW48_18310)	6280..7578	-	1299	WP_119887545.1	MobV family relaxase	-
JHW48_RS18425 (JHW48_18315)	7955..8251	+	297	WP_119887544.1	hypothetical protein	-
JHW48_RS18430 (JHW48_18320)	8438..8857	-	420	WP_170152362.1	GtrA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..9146	9146

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (20-94)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 8696.17 Da
• Isoelectric Point: 8.8786

>T185780 WP_119887537.1 NZ_CP067174:c4527-4279 [Paracoccus aestuarii]
MLMITWDAPKRQSNLAKHGLDFADLDEAFFLASVVVPAKAGRHMAIGRLADGTVAVVFAALGTEGVSVISMRPASAKERS
LL

Nucleotide

• Length: 249 bp

>T185780 NZ_CP067174:c4527-4279 [Paracoccus aestuarii]
ATGCTGATGATCACTTGGGACGCGCCGAAGCGGCAGAGTAACCTTGCCAAGCACGGCTTAGACTTTGCCGATCTAGACGA
AGCCTTCTTTCTGGCCTCCGTCGTGGTTCCAGCGAAAGCAGGCCGGCACATGGCAATCGGTCGCCTCGCAGATGGGACTG
TGGCTGTGGTCTTTGCAGCCTTGGGCACCGAGGGGGTTTCGGTGATCTCGATGCGCCCTGCCAGCGCCAAGGAGAGGAGC
CTGCTATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10675.08 Da
• Isoelectric Point: 8.9128

>AT185780 WP_119887538.1 NZ_CP067174:c4282-3992 [Paracoccus aestuarii]
MSRQHLTEEQIQRMIASDPEAPEATADQLAQARPFTEAFPALSDAMRRNMGGRPKAENPKVAVSLRLDQDVVARFKESGP
GWQTRMNRALREAAGL

Nucleotide

• Length: 291 bp

>AT185780 NZ_CP067174:c4282-3992 [Paracoccus aestuarii]
ATGAGCCGACAGCACCTGACCGAAGAACAGATCCAGCGCATGATTGCCAGCGATCCCGAGGCACCGGAAGCGACGGCGGA
CCAGCTCGCCCAGGCTCGGCCCTTCACCGAGGCCTTCCCAGCCCTGTCCGACGCTATGCGCCGCAACATGGGCGGGCGCC
CCAAGGCCGAGAACCCAAAGGTAGCTGTCTCGCTTCGCCTGGATCAGGACGTGGTGGCCCGGTTCAAGGAAAGCGGTCCA
GGCTGGCAGACCCGGATGAACCGCGCCTTGCGCGAAGCAGCTGGGCTCTGA