Detailed information of TA system TA185779

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 124637..125249
• Replicon: plasmid p296221
• Accession: NZ_CP067170
• Organism: Paracoccus aestuarii strain DSM 19484

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JHW48_RS16190
• Protein ID: WP_119886392.1
• Coordinates: 124854..125249 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JHW48_RS16185
• Protein ID: WP_119886406.1
• Coordinates: 124637..124864 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JHW48_RS16170 (JHW48_16090)	119974..121677	-	1704	WP_119886395.1	potassium-transporting ATPase subunit KdpA	-
JHW48_RS16175 (JHW48_16100)	122222..122638	+	417	WP_119886393.1	response regulator	-
JHW48_RS16180 (JHW48_16105)	122753..124096	-	1344	WP_240637857.1	PAS domain-containing protein	-
JHW48_RS16185 (JHW48_16110)	124637..124864	+	228	WP_119886406.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JHW48_RS16190 (JHW48_16115)	124854..125249	+	396	WP_119886392.1	PIN domain-containing protein	Toxin
JHW48_RS16195 (JHW48_16120)	126443..126829	-	387	WP_147388097.1	hypothetical protein	-
JHW48_RS16200 (JHW48_16125)	126829..127344	-	516	WP_119886390.1	hypothetical protein	-
JHW48_RS16205 (JHW48_16130)	127383..128444	-	1062	WP_272835886.1	hypothetical protein	-
JHW48_RS16210 (JHW48_16135)	128789..129049	+	261	WP_119887966.1	helix-turn-helix transcriptional regulator	-
JHW48_RS16215 (JHW48_16140)	129182..129427	-	246	WP_119887967.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..296221	296221

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-114)

Antitoxin

• SpoVT-AbrB domain (5-46)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14310.46 Da
• Isoelectric Point: 4.4227

>T185779 WP_119886392.1 NZ_CP067170:124854-125249 [Paracoccus aestuarii]
MSVEFVDTNVILYLLDDGPKADRAEAILAQGPRISVQVLNEAMVNCRRKAGMDWNDTASFLAGVRALCPVEDLTLRTHDV
GRALAERYGFSVYDAMIVAAALIAGCETLWTEDMHAGLLVEGQLRLVNPFA

Nucleotide

• Length: 396 bp

>T185779 NZ_CP089024:c5385534-5385151 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTTGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGGTTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8541.81 Da
• Isoelectric Point: 10.6439

>AT185779 WP_119886406.1 NZ_CP067170:124637-124864 [Paracoccus aestuarii]
MQVAKWGNSLAVRLPAELVRDMGLKEGDQIDLLPDDGPLRIRRLPRADEVLDGLRRFRGRLPASERLSRDAAHER

Nucleotide

• Length: 228 bp

>AT185779 NZ_CP089024:c5385776-5385534 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA