Detailed information of TA system TA185777

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 236560..237101
• Replicon: plasmid p242883
• Accession: NZ_CP067141
• Organism: Paracoccus saliphilus strain DSM 18447

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: JHX88_RS22205
• Protein ID: WP_076526808.1
• Coordinates: 236775..237101 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: JHX88_RS22200
• Protein ID: WP_076526807.1
• Coordinates: 236560..236778 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JHX88_RS22175 (JHX88_22105)	232517..234229	-	1713	WP_076526805.1	bifunctional sulfate adenylyltransferase/adenylylsulfate kinase	-
JHX88_RS22180	234580..234918	-	339	Protein_231	transposase	-
JHX88_RS22185 (JHX88_22115)	234966..235721	-	756	Protein_232	IS110 family transposase	-
JHX88_RS22190	235815..236018	+	204	Protein_233	hypothetical protein	-
JHX88_RS22195 (JHX88_22120)	236108..236347	-	240	WP_076526806.1	WGR domain-containing protein	-
JHX88_RS22200 (JHX88_22125)	236560..236778	+	219	WP_076526807.1	antitoxin MazE family protein	Antitoxin
JHX88_RS22205 (JHX88_22130)	236775..237101	+	327	WP_076526808.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JHX88_RS22210 (JHX88_22135)	237226..237630	+	405	WP_076526809.1	GFA family protein	-
JHX88_RS22215 (JHX88_22140)	237782..238479	+	698	Protein_238	IS6 family transposase	-
JHX88_RS22220 (JHX88_22145)	238674..239294	-	621	WP_076526810.1	SMC-Scp complex subunit ScpB	-
JHX88_RS22225 (JHX88_22150)	239297..240169	-	873	WP_272848309.1	DUF1403 family protein	-
JHX88_RS22230 (JHX88_22155)	240296..241393	+	1098	WP_076526811.1	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	katB / glf / glf	1..242883	242883

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-103)

Antitoxin

• Antitoxin MazE-like (7-71)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11497.37 Da
• Isoelectric Point: 4.4930

>T185777 WP_076526808.1 NZ_CP067141:236775-237101 [Paracoccus saliphilus]
VKRGDLVTVALQGDYGKPLPALIIQSDQFDELGSVVILPLTSTLIDAPLLRPTVEPSSDNGLRTPSQIMLDKPMTVKTEK
IGPSFGHLDDLDMIGVSRSLALFLGLAG

Nucleotide

• Length: 327 bp

>T185777 NZ_CP089024:c4749993-4749460 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCTGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8051.05 Da
• Isoelectric Point: 5.0150

>AT185777 WP_076526807.1 NZ_CP067141:236560-236778 [Paracoccus saliphilus]
MPSPVSERVQKRRDALRAAGLRPVQIWVPDTRRPGFAAECRRQAALIAASDRGDADLSDFMDAALNDLDEWQ

Nucleotide

• Length: 219 bp

>AT185777 NZ_CP089024:c4750270-4750004 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA