Detailed information of TA system TA185774

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 10303..11390
• Replicon: plasmid p90204
• Accession: NZ_CP067137
• Organism: Paracoccus fistulariae strain KCTC 22803

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: JHX87_RS17970
• Protein ID: WP_271886982.1
• Coordinates: 10764..11390 (+)
• Length: 209 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: JHX87_RS17965
• Protein ID: WP_271886981.1
• Coordinates: 10303..10767 (+)
• Length: 155 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JHX87_RS17945 (JHX87_17930)	6628..7356	+	729	WP_271886807.1	hypothetical protein	-
JHX87_RS17950 (JHX87_17935)	7458..7859	+	402	WP_271886806.1	hypothetical protein	-
JHX87_RS17955 (JHX87_17940)	8109..9041	-	933	WP_272833967.1	IS110 family transposase	-
JHX87_RS17960 (JHX87_17945)	9344..10127	-	784	Protein_7	IS3 family transposase	-
JHX87_RS17965 (JHX87_17950)	10303..10767	+	465	WP_271886981.1	helix-turn-helix domain-containing protein	Antitoxin
JHX87_RS17970 (JHX87_17955)	10764..11390	+	627	WP_271886982.1	PIN domain-containing protein	Toxin
JHX87_RS17975 (JHX87_17960)	11335..12549	+	1215	WP_272833674.1	IS256 family transposase	-
JHX87_RS17980 (JHX87_17965)	12686..12928	+	243	WP_272833955.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
JHX87_RS17985 (JHX87_17970)	12925..13299	+	375	WP_271886934.1	type II toxin-antitoxin system VapC family toxin	-
JHX87_RS17990 (JHX87_17975)	13691..14077	+	387	Protein_13	IS21 family transposase	-
JHX87_RS17995 (JHX87_17980)	14145..14387	+	243	Protein_14	integrase core domain-containing protein	-
JHX87_RS18000 (JHX87_17985)	14384..15373	-	990	WP_271886933.1	inositol 2-dehydrogenase	-
JHX87_RS18005 (JHX87_17990)	15375..16205	-	831	WP_271886932.1	class II fructose-bisphosphate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..90204	90204

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (11-115)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 209 a.a.
• Molecular weight: 22718.78 Da
• Isoelectric Point: 4.7216

>T185774 WP_271886982.1 NZ_CP067137:10764-11390 [Paracoccus fistulariae]
MSRYTDRFTALLDACVLGGALRRNMLLSLAEAGLFRPRWSSRILDETQKAIAQIIKGATDGSRQRAAIEAAFPEAMVTGY
EIFEDRLALPDPDDNHVLAAAIATSASVIVTDNLADFPFAVLDAHAIQAISADDFIADTIELDPSEAILALRRMRERFKN
PALDVAALIHKSEAQGLLQVGSMLPERSKDHDDFERTAGRAAERLQTA

Nucleotide

• Length: 627 bp

>T185774 NZ_CP089024:2085426-2085528 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 155 a.a.
• Molecular weight: 17023.46 Da
• Isoelectric Point: 4.9044

>AT185774 WP_271886981.1 NZ_CP067137:10303-10767 [Paracoccus fistulariae]
MNALRKEAFFDRLPDEVEIKNAEQLRTIVASQIKEGEPTKLSLALEGGEVQTVTLAPALTASLLEVLRLVSSGRGFRMIP
VESELTTQQAADLLNVSRPFLVKLLEEGKIPFAKTGRHRRVRADDLFAYKNERDASRSDTLSDLAAMDAEEGLV

Nucleotide

• Length: 465 bp

>AT185774 NZ_CP089024:c2085535-2085390 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT