Detailed information of TA system TA185769

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 60213..60805
• Replicon: chromosome
• Accession: NZ_CP067113
• Organism: Luteimonas sp. MC1750

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: JGR68_RS00280
• Protein ID: WP_199363106.1
• Coordinates: 60213..60509 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: JGR68_RS00285
• Protein ID: WP_199363105.1
• Coordinates: 60512..60805 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JGR68_RS00260	55980..56273	+	294	WP_200672708.1	PH domain-containing protein	-
JGR68_RS00265	56970..57503	+	534	WP_199363110.1	hypothetical protein	-
JGR68_RS00270	57623..58129	+	507	WP_199363108.1	hypothetical protein	-
JGR68_RS00275	59429..60100	+	672	WP_199363107.1	PhoU domain-containing protein	-
JGR68_RS00280	60213..60509	+	297	WP_199363106.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JGR68_RS00285	60512..60805	+	294	WP_199363105.1	putative addiction module antidote protein	Antitoxin
JGR68_RS00290	61237..61737	+	501	WP_199363104.1	hypothetical protein	-
JGR68_RS00295	61886..62101	+	216	WP_199363103.1	hypothetical protein	-
JGR68_RS00300	62169..62627	+	459	WP_199363100.1	hypothetical protein	-
JGR68_RS00305	63127..63522	+	396	WP_199363098.1	DUF1801 domain-containing protein	-
JGR68_RS00310	63649..64017	+	369	WP_199363096.1	hypothetical protein	-
JGR68_RS00315	64116..64403	+	288	WP_199392538.1	hypothetical protein	-
JGR68_RS00320	64583..64894	+	312	WP_199363092.1	hypothetical protein	-
JGR68_RS00325	65017..65187	+	171	WP_199363090.1	hypothetical protein	-
JGR68_RS00330	65347..65679	+	333	WP_199363089.1	YnfA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	28874..65187	36313
	-	inside	Integron	-	-	23987..60911	36924

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-87)

Antitoxin

• Uncharacterized protein HI1420 (7-94)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11313.08 Da
• Isoelectric Point: 10.1808

>T185769 WP_199363106.1 NZ_CP067113:60213-60509 [Luteimonas sp. MC1750]
MVEILKSDTFDAWLRALRDRQVKARIEVRIRRLSLENPGQHRALKGGICELKIDHGPGYRVYYTFRNQTLVLLLCGGTKS
TQSQDIKLAQSIAKQWED

Nucleotide

• Length: 297 bp

>T185769 NZ_CP089023:c102788-102318 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10342.82 Da
• Isoelectric Point: 5.8123

>AT185769 WP_199363105.1 NZ_CP067113:60512-60805 [Luteimonas sp. MC1750]
MTKVKEKFSRYDTADYLETEADIAAYLNACAEEDDPALLVAALGDVARARNMTQLAEKTGMTRMGLYKALSGQGNPSFAT
VSKVAHALGLKITVAAT

Nucleotide

• Length: 294 bp

>AT185769 NZ_CP089023:c103228-102785 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA