Detailed information of TA system TA185767

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 6517609..6518146
• Replicon: chromosome
• Accession: NZ_CP067102
• Organism: Bradyrhizobium diazoefficiens strain 36_1

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: JJC00_RS30345
• Protein ID: WP_200469487.1
• Coordinates: 6517889..6518146 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: JJC00_RS30340
• Protein ID: WP_200469486.1
• Coordinates: 6517609..6517902 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJC00_RS30315	6512874..6513725	-	852	WP_200469481.1	3-mercaptopyruvate sulfurtransferase	-
JJC00_RS30320	6513997..6514410	-	414	WP_200469482.1	rhodanese-like domain-containing protein	-
JJC00_RS30325	6514521..6515696	-	1176	WP_200469483.1	VWA domain-containing protein	-
JJC00_RS30330	6515932..6516774	+	843	WP_200469484.1	MoxR family ATPase	-
JJC00_RS30335	6517261..6517578	-	318	WP_200469485.1	immunity 53 family protein	-
JJC00_RS30340	6517609..6517902	-	294	WP_200469486.1	BrnA antitoxin family protein	Antitoxin
JJC00_RS30345	6517889..6518146	-	258	WP_200469487.1	BrnT family toxin	Toxin
JJC00_RS30350	6518311..6519957	-	1647	WP_200469488.1	GMC family oxidoreductase N-terminal domain-containing protein	-
JJC00_RS30355	6519974..6520567	-	594	WP_200469489.1	GNAT family N-acetyltransferase	-
JJC00_RS30360	6520772..6521710	-	939	WP_200469490.1	SMP-30/gluconolactonase/LRE family protein	-
JJC00_RS30365	6521849..6522682	-	834	WP_200469491.1	AraC family transcriptional regulator	-
JJC00_RS30370	6522770..6523123	-	354	WP_200469492.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (20-92)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9835.48 Da
• Isoelectric Point: 10.3736

>T185767 WP_200469487.1 NZ_CP067102:c6518146-6517889 [Bradyrhizobium diazoefficiens]
MKIVWDEPKRLANLDKHGLDFADLNETFFENALVVPSYKDKRWVAICKSIRGVIVVVFARLGREGVSIISMRPASRSERR
LYAER

Nucleotide

• Length: 258 bp

>T185767 NZ_CP089022:58677-58988 [Klebsiella pneumoniae]
GTGCATGTTATATCACGAGCCCCTTTTGATGAGGCGGCGAGACACTATCCGAATGATGCTGCGGCCATCGATGACACCTA
TCGAGTACTAAAGCGTGTTGTCGCAAAAAAACCGGATGAGCTAAAGCGATACTTTACTAGTCTGGATCGCATGAAATACC
GGGAAAAATGGTGGGTTATCGATATCGGCGGTAATAACCTTAGAATGATGTTTTTTGCTGATTTTGAGCGCGGAAAGATA
TTCGTCAAACACATAACCACCCATGCTGAATATGATCGCTTAACGAAATATTATCGGGAGCACAAAGAATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10893.52 Da
• Isoelectric Point: 11.2412

>AT185767 WP_200469486.1 NZ_CP067102:c6517902-6517609 [Bradyrhizobium diazoefficiens]
MPNAKKKSGYTKRDLREVSDNPELTKADFAKARPFSEVFPDLSASIRRGRGPNKAPTKKLVSLRLSPEVVEHFKSTGPGW
QSRIDETLRKAVKRKAS

Nucleotide

• Length: 294 bp

>AT185767 NZ_CP089022:58985-59401 [Klebsiella pneumoniae]
ATGATTTTTAGCGATGCCATTAAAGCGGCGAATGACCTGGCGAGTATTGTCCCTCTGCTGGGTGGCAGCTCGTCCCGCAA
AGATTACGAAGAGGCGCTTAAACTCGTTGAATATCTTCTGGAACATGACCCGGACAGCCCGCTGGTTGATATGCTGACTG
CCCGCATCGATGCCTGGGAAGATAACGCGGTGGAATTTGAAGAATTTAATACGCGCATCGAAGCAGGCAAAAACGGTGTA
TCGTTGCTGCGGGTTCTTATGCAGCAACACGGGCTTTCACAGTCCGATTTTGAAAACGAGATAGGCAAAAAATCGCTCGT
CAGCCGGATCCTCAGTGGTGAACGCAGTCTGACCCTTGATCACATGCGGGCGCTGGCAAATCGATTTCAAATCCCGGTCT
CGATGTTTGTTGACTGA