Detailed information of TA system TA185766

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1001772..1002364
• Replicon: chromosome
• Accession: NZ_CP067102
• Organism: Bradyrhizobium diazoefficiens strain 36_1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: JJC00_RS04660
• Protein ID: WP_200471573.1
• Coordinates: 1002176..1002364 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JJC00_RS04655
• Protein ID: WP_200473977.1
• Coordinates: 1001772..1002170 (-)
• Length: 133 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJC00_RS04635	997742..998161	-	420	WP_200471569.1	acyl-CoA thioesterase	-
JJC00_RS04640	998173..998934	-	762	WP_200471570.1	SDR family NAD(P)-dependent oxidoreductase	-
JJC00_RS04645	998957..1000840	-	1884	WP_200471571.1	feruloyl-CoA synthase	-
JJC00_RS04650	1000837..1001646	-	810	WP_200471572.1	crotonase/enoyl-CoA hydratase family protein	-
JJC00_RS04655	1001772..1002170	-	399	WP_200473977.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
JJC00_RS04660	1002176..1002364	-	189	WP_200471573.1	type II toxin-antitoxin system HicA family toxin	Toxin
JJC00_RS04665	1002409..1002870	-	462	WP_200471574.1	DUF3237 domain-containing protein	-
JJC00_RS04670	1002883..1004202	-	1320	WP_200471575.1	TRAP transporter permease	-
JJC00_RS04675	1004205..1004714	-	510	WP_200473978.1	TRAP transporter small permease	-
JJC00_RS04680	1004942..1005958	-	1017	WP_200471576.1	TRAP transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-58)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-124)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7043.17 Da
• Isoelectric Point: 11.4111

>T185766 WP_200471573.1 NZ_CP067102:c1002364-1002176 [Bradyrhizobium diazoefficiens]
MNSREIISALQRDGWVQVAQKGSHVQFKHPTKTGRVTVPHPKRDIPLGTLKSIEKQSDLKLR

Nucleotide

• Length: 189 bp

>T185766 NZ_CP089022:c36153-35866 [Klebsiella pneumoniae]
ATGACATATACCGTCAAATTCAGGGAAGATGCGCTAAAAGAATGGAACAAGCTGGATAAGACTATTCAGCAGCAGTTTGC
AAAGAAACTTAAAAAGTGCTGTGAAAACCCACACATTCCTTCAGCAAAACTACGTGGTATAAAGGATTGCTATAAAATAA
AACTGCGAGCATCTGGTTTTCGCCTCGTCTATCAGGTCATAGATAATCAACTTATAATTGCTATTGTGGCTGTAGGCAAA
CGGGAGCGAAGTGACGTTTATACACTGGCGAGTGAACGTATGAAATAA

Antitoxin

Protein

• Length: 133 a.a.
• Molecular weight: 14248.06 Da
• Isoelectric Point: 4.3695

>AT185766 WP_200473977.1 NZ_CP067102:c1002170-1001772 [Bradyrhizobium diazoefficiens]
MRHYIGLIHKDADSDFGVSFPDLPGVVTAGTTLDDARDMAEEALALHLEGMAEDGEAIPEPSSLEEIMSDPKNRSGVAIL
VSVKNDQPKIVRVNVTLPGDVLEQIDKYAETHGYTRSGLLTQAAKKMITETA

Nucleotide

• Length: 399 bp

>AT185766 NZ_CP089022:c36401-36150 [Klebsiella pneumoniae]
ATGCCACATATCATTCTGAGTGACACGACTGCCAGCGTCAGTGAATTGAAAAAAAACCCGATGGCCACAGTCAGCGCCGG
TGACGGGTTCCCAGTAGCGATTCTTAACCGGAACCAGCCAGCTTTCTATTGCGTGCCTGCGGAACTCTACGAAAAGATGC
TTGATGCGCTGGATGATCAGGAACTCATTAAACTGGTTGCAGAACGCAGTAATCAACCGCTGTTTGATGTGGACCTGGAT
AAGTACCTATGA