Detailed information of TA system TA185765

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 641490..642121
• Replicon: chromosome
• Accession: NZ_CP067102
• Organism: Bradyrhizobium diazoefficiens strain 36_1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JJC00_RS02985
• Protein ID: WP_200471275.1
• Coordinates: 641717..642121 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JJC00_RS02980
• Protein ID: WP_200471274.1
• Coordinates: 641490..641720 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJC00_RS02965	637905..638697	-	793	Protein_586	hypothetical protein	-
JJC00_RS02970	638846..639862	+	1017	WP_200471273.1	LysM peptidoglycan-binding domain-containing protein	-
JJC00_RS02975	640048..641331	+	1284	WP_200473962.1	Spy/CpxP family protein refolding chaperone	-
JJC00_RS02980	641490..641720	+	231	WP_200471274.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JJC00_RS02985	641717..642121	+	405	WP_200471275.1	PIN domain-containing protein	Toxin
JJC00_RS02990	642249..645404	-	3156	WP_200471276.1	type I restriction endonuclease subunit R	-
JJC00_RS02995	645401..645643	-	243	WP_200471277.1	hypothetical protein	-
JJC00_RS03000	645640..646992	-	1353	WP_200471278.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-114)

Antitoxin

• SpoVT-AbrB domain (5-45)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14572.64 Da
• Isoelectric Point: 6.2087

>T185765 WP_200471275.1 NZ_CP067102:641717-642121 [Bradyrhizobium diazoefficiens]
VSAFFDSNILIYAYSTDIRRQRALTTIAGGGVISAQVLNEFTNVLRKKQKQDWSIIEAAVQSIRFRFPDIVPLTSDTHAA
ALVLARDHGLAFYDALIVTCAIDAGCDTLYSEDMQHGRSIGGLAIVNPFLGSVP

Nucleotide

• Length: 405 bp

>T185765 NZ_CP089021:c5401533-5401150 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTTGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGGTTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8687.92 Da
• Isoelectric Point: 6.3540

>AT185765 WP_200471274.1 NZ_CP067102:641490-641720 [Bradyrhizobium diazoefficiens]
MQVSKWGNSLAVRLPKALVEALKLSPGDELDVVEASKGRIAVEKVDKRAEFLRQVEQFRFPLPDGYKFDRDEANER

Nucleotide

• Length: 231 bp

>AT185765 NZ_CP089021:c5401775-5401533 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA