Detailed information of TA system TA185764

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii(toxin)
• Location: 6374337..6375171
• Replicon: chromosome
• Accession: NZ_CP067101
• Organism: Bradyrhizobium diazoefficiens strain 41_2

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: JJB98_RS29900
• Protein ID: WP_200456855.1
• Coordinates: 6374337..6374645 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: JJB98_RS29905
• Protein ID: WP_200456856.1
• Coordinates: 6374638..6375171 (-)
• Length: 178 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB98_RS29880	6371035..6371568	+	534	WP_200456852.1	sarcosine oxidase subunit gamma	-
JJB98_RS29885	6371642..6372529	+	888	WP_200456853.1	formyltetrahydrofolate deformylase	-
JJB98_RS29890	6372531..6373415	+	885	Protein_5931	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD	-
JJB98_RS29895	6374017..6374199	+	183	WP_200456854.1	hypothetical protein	-
JJB98_RS29900	6374337..6374645	-	309	WP_200456855.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
JJB98_RS29905	6374638..6375171	-	534	WP_200456856.1	transcriptional regulator	Antitoxin
JJB98_RS29910	6375654..6375974	-	321	WP_200456857.1	DUF736 domain-containing protein	-
JJB98_RS29915	6376533..6377009	-	477	WP_200456858.1	hypothetical protein	-
JJB98_RS29920	6377242..6378174	-	933	WP_200456859.1	DUF2493 domain-containing protein	-
JJB98_RS29925	6378978..6379295	-	318	WP_200456860.1	QacE family quaternary ammonium compound efflux SMR transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	6334051..6379295	45244

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (38-79)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11838.00 Da
• Isoelectric Point: 11.1059

>T185764 WP_200456855.1 NZ_CP067101:c6374645-6374337 [Bradyrhizobium diazoefficiens]
MRKPLRNVGASVRGRLLNVSKERNEPYELLLTQYALERLLYRLSISKYKEQFALKGAMLLRYWLHDRHRPTRDLDLLGFG
KSGPQLTLGSPKNIVKLKLSMA

Nucleotide

• Length: 309 bp

>T185764 NZ_CP089021:c4931525-4931241 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 178 a.a.
• Molecular weight: 20080.15 Da
• Isoelectric Point: 10.0613

>AT185764 WP_200456856.1 NZ_CP067101:c6375171-6374638 [Bradyrhizobium diazoefficiens]
MEPQRQEDRATSLLRARGMMRLSEFLNKISRRQPFPGWSVVCYDSALAFHELTDHIPRSVWMAIGPRDWRSKITSPRIEI
VRFGPKEFDKGVEHHTIEGVPVPIYTPAKTIIDLFKGGQRPKAFHNAPAGIAHATQAMKDALRLHKATPSEIAEYAVDAG
IWQKIVQPRFETLTIDA

Nucleotide

• Length: 534 bp

>AT185764 NZ_CP089021:c4931757-4931515 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA