Detailed information of TA system TA185762

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 4350111..4350696
• Replicon: chromosome
• Accession: NZ_CP067101
• Organism: Bradyrhizobium diazoefficiens strain 41_2

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: JJB98_RS20560
• Protein ID: WP_200455275.1
• Coordinates: 4350400..4350696 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: JJB98_RS20555
• Protein ID: WP_200455274.1
• Coordinates: 4350111..4350398 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB98_RS20535	4345997..4346632	-	636	WP_200455271.1	ATP-dependent Clp protease proteolytic subunit	-
JJB98_RS20540	4347024..4348385	-	1362	WP_200455272.1	trigger factor	-
JJB98_RS20550	4348798..4350102	+	1305	WP_200455273.1	multicopper oxidase domain-containing protein	-
JJB98_RS20555	4350111..4350398	-	288	WP_200455274.1	putative addiction module antidote protein	Antitoxin
JJB98_RS20560	4350400..4350696	-	297	WP_200455275.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JJB98_RS20565	4350745..4351169	-	425	Protein_4076	GNAT family N-acetyltransferase	-
JJB98_RS20570	4351169..4352668	-	1500	WP_200455276.1	NAD(P)H-hydrate dehydratase	-
JJB98_RS20575	4353050..4353388	+	339	WP_007603495.1	P-II family nitrogen regulator	-
JJB98_RS20580	4353540..4354949	+	1410	WP_200455277.1	type I glutamate--ammonia ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-92)

Antitoxin

• Uncharacterized protein HI1420 (4-92)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10910.59 Da
• Isoelectric Point: 10.4088

>T185762 WP_200455275.1 NZ_CP067101:c4350696-4350400 [Bradyrhizobium diazoefficiens]
VVELKQTETFRKWRSRLKDERAKALIASRLDRLALGHAGDVEPVGGGISELRIHHGPGYRIYFQKQGGLIIVLLCGGDKS
TQAKDIKAAKRLAETWSE

Nucleotide

• Length: 297 bp

>T185762 NZ_CP089021:4173015-4173233 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10303.76 Da
• Isoelectric Point: 5.1023

>AT185762 WP_200455274.1 NZ_CP067101:c4350398-4350111 [Bradyrhizobium diazoefficiens]
MGEKLTTYDPAEDLQSDEAIAVFMEEAFKTEDAAYIAHALGVVARAKGMTQIAKETGLSREQLYRSFSTNGNPTLKTTIA
VMKALGIELTAKPHV

Nucleotide

• Length: 288 bp

>AT185762 NZ_CP089021:4172614-4172988 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG