Detailed information of TA system TA185760

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 3745923..3746511
• Replicon: chromosome
• Accession: NZ_CP067101
• Organism: Bradyrhizobium diazoefficiens strain 41_2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: JJB98_RS17710
• Protein ID: WP_200454776.1
• Coordinates: 3746158..3746511 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: JJB98_RS17705
• Protein ID: WP_200454775.1
• Coordinates: 3745923..3746177 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB98_RS17670	3741861..3742211	-	351	WP_200454769.1	alkylphosphonate utilization protein	-
JJB98_RS17675	3742338..3742511	-	174	WP_200454770.1	hypothetical protein	-
JJB98_RS17680	3742789..3743193	+	405	WP_200454771.1	VOC family protein	-
JJB98_RS17685	3743249..3743914	+	666	WP_200454772.1	cell envelope biogenesis protein TolA	-
JJB98_RS17690	3743955..3744178	+	224	Protein_3511	helix-turn-helix transcriptional regulator	-
JJB98_RS17695	3744457..3744738	+	282	WP_200454773.1	hypothetical protein	-
JJB98_RS17700	3744920..3745783	-	864	WP_200454774.1	nitroreductase family protein	-
JJB98_RS17705	3745923..3746177	+	255	WP_200454775.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JJB98_RS17710	3746158..3746511	+	354	WP_200454776.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JJB98_RS17715	3746766..3747053	+	288	WP_200454777.1	hypothetical protein	-
JJB98_RS17720	3747352..3747867	-	516	WP_200454778.1	ParB N-terminal domain-containing protein	-
JJB98_RS17725	3748104..3749000	-	897	WP_200454779.1	LysR family transcriptional regulator	-
JJB98_RS17730	3749142..3749542	+	401	Protein_3519	cupin domain-containing protein	-
JJB98_RS17735	3749552..3749763	+	212	Protein_3520	aldo/keto reductase	-
JJB98_RS17740	3749857..3751260	-	1404	WP_200454780.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-115)

Antitoxin

• Antitoxin PemI-like (1-84)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 12539.36 Da
• Isoelectric Point: 6.7026

>T185760 WP_200454776.1 NZ_CP067101:3746158-3746511 [Bradyrhizobium diazoefficiens]
MATKSGDHCPEAGDFIWIDLDPTVGHEQSGRRPAIVVSPRSYNAAAGLCVICPITSRVRGYPFEAVLPEGAGLRGAVLAD
QPRSISWEKRPIAHAGRAPDILLTEVRERLAALLGID

Nucleotide

• Length: 354 bp

>T185760 NZ_CP089021:2092137-2092239 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9413.80 Da
• Isoelectric Point: 9.8617

>AT185760 WP_200454775.1 NZ_CP067101:3745923-3746177 [Bradyrhizobium diazoefficiens]
MKVAFQKWGNSLALRVPKAFADEIGARDGRAAEMTVNNGKLVIEIALPKRRKRRYALDELVAGITPDNRHDEIDWGPAVG
NEVW

Nucleotide

• Length: 255 bp

>AT185760 NZ_CP089021:c2092246-2092101 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT