Detailed information of TA system TA185758

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 1878089..1878629
• Replicon: chromosome
• Accession: NZ_CP067101
• Organism: Bradyrhizobium diazoefficiens strain 41_2

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: JJB98_RS08825
• Protein ID: WP_200453163.1
• Coordinates: 1878089..1878349 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: JJB98_RS08830
• Protein ID: WP_200453164.1
• Coordinates: 1878336..1878629 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB98_RS08805	1873408..1874241	+	834	WP_200453159.1	AraC family transcriptional regulator	-
JJB98_RS08810	1874378..1875304	+	927	WP_200453160.1	SMP-30/gluconolactonase/LRE family protein	-
JJB98_RS08815	1875444..1876037	+	594	WP_200453161.1	GNAT family N-acetyltransferase	-
JJB98_RS08820	1876269..1877915	+	1647	WP_200453162.1	GMC family oxidoreductase N-terminal domain-containing protein	-
JJB98_RS08825	1878089..1878349	+	261	WP_200453163.1	BrnT family toxin	Toxin
JJB98_RS08830	1878336..1878629	+	294	WP_200453164.1	BrnA antitoxin family protein	Antitoxin
JJB98_RS08835	1878747..1879085	+	339	WP_200453165.1	immunity 53 family protein	-
JJB98_RS08840	1879958..1880800	-	843	WP_200453166.1	MoxR family ATPase	-
JJB98_RS08845	1880894..1881136	-	243	WP_200453167.1	GlsB/YeaQ/YmgE family stress response membrane protein	-
JJB98_RS08850	1881260..1882435	+	1176	WP_200453168.1	VWA domain-containing protein	-
JJB98_RS08855	1882564..1882977	+	414	WP_200453169.1	rhodanese-like domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-79)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (20-92)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9663.18 Da
• Isoelectric Point: 10.5537

>T185758 WP_200453163.1 NZ_CP067101:1878089-1878349 [Bradyrhizobium diazoefficiens]
MKIVWDEPKRLANLDKHGLDFADLNETFFDNALVVPSHNKSKRWVAVGVSISGVIVVVFARLGREGVSIISVRPASRSER
KLYAER

Nucleotide

• Length: 261 bp

>T185758 NZ_CP089021:732143-732628 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCGCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATTCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10824.41 Da
• Isoelectric Point: 11.1114

>AT185758 WP_200453164.1 NZ_CP067101:1878336-1878629 [Bradyrhizobium diazoefficiens]
MPNARKRSGYTKEDLRAVSDNPELTKADFAKAKPFSEVFPDLSASIRKGRGPNKSPTKKLVSLRLSPEVVEHFKSTGPGW
QSRIDETLRKAVKRKAS

Nucleotide

• Length: 294 bp

>AT185758 NZ_CP089021:731853-732146 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA