Detailed information of TA system TA185757

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1410415..1411007
• Replicon: chromosome
• Accession: NZ_CP067101
• Organism: Bradyrhizobium diazoefficiens strain 41_2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: JJB98_RS06650
• Protein ID: WP_200452776.1
• Coordinates: 1410819..1411007 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JJB98_RS06645
• Protein ID: WP_200452775.1
• Coordinates: 1410415..1410813 (-)
• Length: 133 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB98_RS06625	1406360..1406779	-	420	WP_200452771.1	acyl-CoA thioesterase	-
JJB98_RS06630	1406791..1407552	-	762	WP_200452772.1	SDR family NAD(P)-dependent oxidoreductase	-
JJB98_RS06635	1407580..1409463	-	1884	WP_200452773.1	feruloyl-CoA synthase	-
JJB98_RS06640	1409460..1410269	-	810	WP_200452774.1	crotonase/enoyl-CoA hydratase family protein	-
JJB98_RS06645	1410415..1410813	-	399	WP_200452775.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
JJB98_RS06650	1410819..1411007	-	189	WP_200452776.1	type II toxin-antitoxin system HicA family toxin	Toxin
JJB98_RS06655	1411052..1411513	-	462	WP_200452777.1	DUF3237 domain-containing protein	-
JJB98_RS06660	1411525..1412844	-	1320	WP_200452778.1	TRAP transporter permease	-
JJB98_RS06665	1412847..1413356	-	510	WP_200452779.1	TRAP transporter small permease	-
JJB98_RS06670	1413553..1414569	-	1017	WP_200452780.1	TRAP transporter substrate-binding protein	-
JJB98_RS06675	1414833..1416002	+	1170	WP_200452781.1	4-hydroxybenzoate 3-monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-124)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 6911.06 Da
• Isoelectric Point: 11.7334

>T185757 WP_200452776.1 NZ_CP067101:c1411007-1410819 [Bradyrhizobium diazoefficiens]
MNSREIIAALQGDGWVQVAQKGSHVQFKHPTKKGRVTVPHPKRDVPLGTLKSIERQSGLKLR

Nucleotide

• Length: 189 bp

>T185757 NZ_CP089021:327907-328275 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACTGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 133 a.a.
• Molecular weight: 14296.16 Da
• Isoelectric Point: 4.4569

>AT185757 WP_200452775.1 NZ_CP067101:c1410813-1410415 [Bradyrhizobium diazoefficiens]
MRHYIGLIHKDANSDFGVLFPDLPGLVTAGTTLDEARDMAEEALAFHLEGMAEDGEAIPEPSSLEEIMSNPENRSGVAIL
VSVKDHQPKIVRVNVTLPGDVLEQIDRYAEAHGYTRSGLLAQAAKKLMTEAA

Nucleotide

• Length: 399 bp

>AT185757 NZ_CP089021:327689-327910 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA