Detailed information of TA system TA185756

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii(toxin)
• Location: 6885842..6887348
• Replicon: chromosome
• Accession: NZ_CP067100
• Organism: Bradyrhizobium diazoefficiens strain 38_8

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: JJB99_RS32460
• Protein ID: WP_200496195.1
• Coordinates: 6885842..6886714 (-)
• Length: 291 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: JJB99_RS32465
• Protein ID: WP_200496196.1
• Coordinates: 6886707..6887348 (-)
• Length: 214 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB99_RS32435	6881706..6881863	-	158	Protein_6441	DUF736 domain-containing protein	-
JJB99_RS32440	6882039..6882635	+	597	WP_200500406.1	DUF2384 domain-containing protein	-
JJB99_RS32445	6882635..6883096	+	462	WP_200496192.1	RES family NAD+ phosphorylase	-
JJB99_RS32450	6883326..6883646	-	321	WP_200496193.1	DUF736 domain-containing protein	-
JJB99_RS32455	6884630..6885754	+	1125	WP_200496194.1	Fic family protein	-
JJB99_RS32460	6885842..6886714	-	873	WP_200496195.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
JJB99_RS32465	6886707..6887348	-	642	WP_200496196.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
JJB99_RS32470	6887824..6887984	-	161	Protein_6448	DUF736 domain-containing protein	-
JJB99_RS32475	6888338..6888816	+	479	Protein_6449	hypothetical protein	-
JJB99_RS32480	6888865..6889941	+	1077	WP_200496197.1	cell division protein FtsZ	-
JJB99_RS32485	6890395..6890715	-	321	WP_200496198.1	DUF736 domain-containing protein	-
JJB99_RS32490	6891201..6891647	-	447	WP_200496199.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	6865529..6893931	28402

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (38-264)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (12-55)

Sequences

Toxin

Protein

• Length: 291 a.a.
• Molecular weight: 33020.87 Da
• Isoelectric Point: 7.8706

>T185756 WP_200496195.1 NZ_CP067100:c6886714-6885842 [Bradyrhizobium diazoefficiens]
MRKPLKNVGASVRARLLNLSKERNEPYDLLLTQYALERLLYRLSISKYKEQFALKGAMLLRYWLHDPHRPTRDLDLLGFG
ESDPQLTRGFFKEICEAQAVDGVTFDTGSLEVDRVRDESGYGGLRLKCYATIDGARLRIVIDIGYGDATEPGLNEIELLT
LLDQPAPKLRAYPPETVIAEKFQAMVHLGLANTRLKDFYDIWVLTRTYEFKDDRLSRAIKATFTRRKTNIPEDRPDALTK
AFTDDATKVQQWNAFVQDVAIDPGPLADVVTTLGTFLMPHAEKARKLKDN

Nucleotide

• Length: 873 bp

>T185756 NZ_CP089020:c139241-138828 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGTAG

Antitoxin

Protein

• Length: 214 a.a.
• Molecular weight: 23963.76 Da
• Isoelectric Point: 10.3238

>AT185756 WP_200496196.1 NZ_CP067100:c6887348-6886707 [Bradyrhizobium diazoefficiens]
MEPQRQEDRATSLLRARGMMRLSEFLNEGITAATISRMERKGALNQLARGLYQLPDALLDGNHTLAVAAKLVPIGVVCYD
SALAFHELTDRIPRSVWMAIRPRDWRPKITRPRIEIVRFGPKEFDRGVEHHTIEGVSVPIYTPAKTIVDLFKSGQRQKAF
YNTPAGITHATQAMKDALRLHKATPSEIAKYAVDAGIWQKLVQPRLETLTIDA

Nucleotide

• Length: 642 bp

>AT185756 NZ_CP089020:c139468-139238 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA