Detailed information of TA system TA185755

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 4408663..4409251
• Replicon: chromosome
• Accession: NZ_CP067100
• Organism: Bradyrhizobium diazoefficiens strain 38_8

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: JJB99_RS20915
• Protein ID: WP_200494218.1
• Coordinates: 4408663..4409016 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: JJB99_RS20920
• Protein ID: WP_200494219.1
• Coordinates: 4408997..4409251 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB99_RS20890	4404287..4406602	+	2316	WP_210347623.1	hypothetical protein	-
JJB99_RS20895	4406825..4407340	+	516	WP_200494214.1	ParB N-terminal domain-containing protein	-
JJB99_RS20900	4407424..4407690	-	267	WP_200494215.1	DUF2312 domain-containing protein	-
JJB99_RS20905	4407802..4407939	+	138	WP_200494216.1	hypothetical protein	-
JJB99_RS20910	4407978..4408637	-	660	WP_200494217.1	cell envelope biogenesis protein TolA	-
JJB99_RS20915	4408663..4409016	-	354	WP_200494218.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JJB99_RS20920	4408997..4409251	-	255	WP_200494219.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JJB99_RS20925	4409443..4410450	-	1008	WP_200494220.1	acyl-CoA desaturase	-
JJB99_RS20930	4410721..4411002	-	282	WP_200494221.1	hypothetical protein	-
JJB99_RS20935	4411312..4411848	-	537	WP_200494222.1	helix-turn-helix domain-containing protein	-
JJB99_RS20940	4411976..4412374	-	399	WP_200494223.1	VOC family protein	-
JJB99_RS20945	4412387..4413793	-	1407	WP_200494224.1	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (12-113)

Antitoxin

• SpoVT-AbrB domain (6-44)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 12672.59 Da
• Isoelectric Point: 5.1526

>T185755 WP_200494218.1 NZ_CP067100:c4409016-4408663 [Bradyrhizobium diazoefficiens]
MATKPGDYCPEAGDVIWIDLDPTVGHEQRGRRPAIVVSPRSYNAVAGLCVICPITSRVRGYPFEAVLPEDASVRGVVLAD
QPRSISWEKRPITPAGRAPDALLLEVREQLAALLGIE

Nucleotide

• Length: 354 bp

>T185755 NZ_CP089020:c88785-88315 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9363.65 Da
• Isoelectric Point: 9.9201

>AT185755 WP_200494219.1 NZ_CP067100:c4409251-4408997 [Bradyrhizobium diazoefficiens]
MKVAFQKWGNSLALRVPKAFADEIGARDGRAAEMTVSEGKLVIEIARAQRRKRRYALDDLVAGITQANRHDEIDWGPAVG
NEAW

Nucleotide

• Length: 255 bp

>AT185755 NZ_CP089020:c89225-88782 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGATCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA