Detailed information of TA system TA185754

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 6446762..6448389
• Replicon: chromosome
• Accession: NZ_CP067098
• Organism: Pseudomonas sp. SW-3

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: JIO00_RS29295
• Protein ID: WP_200667315.1
• Coordinates: 6447037..6448389 (-)
• Length: 451 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: JIO00_RS29290
• Protein ID: WP_083375432.1
• Coordinates: 6446762..6447040 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JIO00_RS29270	6443125..6444675	-	1551	WP_169857211.1	PLP-dependent aminotransferase family protein	-
JIO00_RS29275	6444766..6445389	+	624	WP_010465356.1	FMN-binding negative transcriptional regulator	-
JIO00_RS29280	6445399..6445845	+	447	WP_083375434.1	GNAT family N-acetyltransferase	-
JIO00_RS29285	6445863..6446534	+	672	WP_200667314.1	GNAT family N-acetyltransferase	-
JIO00_RS29290	6446762..6447040	-	279	WP_083375432.1	helix-turn-helix domain-containing protein	Antitoxin
JIO00_RS29295	6447037..6448389	-	1353	WP_200667315.1	HipA domain-containing protein	Toxin
JIO00_RS29300	6448622..6451585	+	2964	WP_200667316.1	autotransporter domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (165-376)

Antitoxin

• Cro/C1-type helix-turn-helix domain (27-80)

Sequences

Toxin

Protein

• Length: 451 a.a.
• Molecular weight: 50404.02 Da
• Isoelectric Point: 5.2652

>T185754 WP_200667315.1 NZ_CP067098:c6448389-6447037 [Pseudomonas sp. SW-3]
MYDLTLQIFITGKWHDAMVLSFDSPEKGFESRCNFGYATPYLIENIEDIGAPFAKAVSARFPLEWEGKRSSAPAFLHDIA
PSGAAKRFLLARIGRDKPTDISTDLYLVARSTPAPIGNMRIKESVEAVDQREAIGFKRQEVISRDNRFLEYAYEQGAAIG
GATGAGGEAPKLLLVQGKDGNLYPDAVLDDADVTQHWFVKFSRNKGNRNDQDILRSEYHYYKALQKLDIETVAEQGLALE
EATKPSLWMQRFDRQVTPQGVERFAVESIYSLAQITTPGSAMDHMEVIRLLARLWREAGQTDQIPNLVADYLRRDLINKI
LGNSDNHGRNTAILRDTASFRLAPIYDLAPMVMDDEGVTRTTKWPKELERAGDVDWRGVCRALADIADPNDPLAAVSDAS
NSFERLREDAKHLAALPDILTASGLPDATMNHPRIALKNLEQRLKEWDLK

Nucleotide

• Length: 1353 bp

>T185754 NZ_CP089020:47286-47768 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCACTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGAAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCTACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGTGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGCGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10259.02 Da
• Isoelectric Point: 10.5916

>AT185754 WP_083375432.1 NZ_CP067098:c6447040-6446762 [Pseudomonas sp. SW-3]
MSMTVAERTVLIESIQEGLAQGTLEIGEAVRRLRVEATGLHQTQFAKMCKISVRTLVHIEHGEGNQTLKSLNAVFKPFGL
KMGVVRIRRDLN

Nucleotide

• Length: 279 bp

>AT185754 NZ_CP089020:47032-47298 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGTACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGATTCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA