Detailed information of TA system TA185749

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 6933..7576
• Replicon: plasmid p2
• Accession: NZ_CP067095
• Organism: Klebsiella michiganensis strain B106

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: IMZ21_RS29310
• Protein ID: WP_016236302.1
• Coordinates: 6933..7349 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: IMZ21_RS29315
• Protein ID: WP_001261282.1
• Coordinates: 7346..7576 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMZ21_RS29290	2301..2651	+	351	WP_004187110.1	DUF305 domain-containing protein	-
IMZ21_RS29295	2837..3745	-	909	WP_032451458.1	HNH endonuclease	-
IMZ21_RS29300	4290..5312	-	1023	WP_000361404.1	helicase UvrD	-
IMZ21_RS29305	5297..6859	-	1563	WP_004206609.1	AAA family ATPase	-
IMZ21_RS29310	6933..7349	-	417	WP_016236302.1	type II toxin-antitoxin system VapC family toxin	Toxin
IMZ21_RS29315	7346..7576	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
IMZ21_RS29320	7533..7955	+	423	WP_160887026.1	hypothetical protein	-
IMZ21_RS29325	8495..8926	+	432	WP_174805835.1	hypothetical protein	-
IMZ21_RS29330	9544..10998	-	1455	WP_200483186.1	EAL domain-containing protein	-
IMZ21_RS29335	11603..11860	+	258	WP_023292103.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..189346	189346

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15091.55 Da
• Isoelectric Point: 7.1084

>T185749 WP_016236302.1 NZ_CP067095:c7349-6933 [Klebsiella michiganensis]
VNKTYMLDTCICSFIMREQPEAVLKRLELAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T185749 NZ_CP089014:c4825310-4825026 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT185749 WP_001261282.1 NZ_CP067095:c7576-7346 [Klebsiella michiganensis]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT185749 NZ_CP089014:c4825542-4825300 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GZG3