Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1737953..1738610 Replicon chromosome
Accession NZ_CP067093
Organism Klebsiella michiganensis strain B106

Toxin (Protein)


Gene name cptA Uniprot ID H3N294
Locus tag IMZ21_RS08300 Protein ID WP_004124948.1
Coordinates 1738200..1738610 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID H3N295
Locus tag IMZ21_RS08295 Protein ID WP_004124953.1
Coordinates 1737953..1738219 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMZ21_RS08280 1733208..1733633 - 426 WP_004124962.1 PTS sugar transporter subunit IIA -
IMZ21_RS08285 1733754..1736552 - 2799 WP_123828836.1 transcriptional regulator DagR -
IMZ21_RS08290 1736725..1737708 - 984 WP_004124956.1 tRNA-modifying protein YgfZ -
IMZ21_RS08295 1737953..1738219 + 267 WP_004124953.1 FAD assembly factor SdhE Antitoxin
IMZ21_RS08300 1738200..1738610 + 411 WP_004124948.1 protein YgfX Toxin
IMZ21_RS08305 1738619..1739140 - 522 WP_004124946.1 flavodoxin FldB -
IMZ21_RS08310 1739262..1740158 + 897 WP_004124942.1 site-specific tyrosine recombinase XerD -
IMZ21_RS08315 1740181..1740894 + 714 WP_123828837.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
IMZ21_RS08320 1740900..1742633 + 1734 WP_004124937.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-132)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16089.88 Da        Isoelectric Point: 10.9455

>T185737 WP_004124948.1 NZ_CP067093:1738200-1738610 [Klebsiella michiganensis]
VVLWQSDLRISWRAQWFSLLMHGVVAALVLVLPWPLSYTPLWLILLSLVVFDCVRSQRRIHARQGEIKLLIDSRLRWQKA
EWDIIGTPWVINSGMLLRLRNTENQRTQHLWVAADSMDAGEWRDLRRLVLQKPTQE

Download         Length: 411 bp

>T185737 NZ_CP089013:35033-35503 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10411.88 Da        Isoelectric Point: 5.1296

>AT185737 WP_004124953.1 NZ_CP067093:1737953-1738219 [Klebsiella michiganensis]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDTLSDGDKALFIRLLENDDPDLFNWLMNHGEPADAELQRMVKLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT185737 NZ_CP089013:34593-35036 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB H3N294

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H3L9

References